Maternal stress in early pregnancy: the reason of endocrine and behaviour disorders in female offspring
Lorianna Sergienko, Oksana Kartavtseva, Tatyana Bondarenko, Olena Perets & Ganna Cherevko
Objective: It is known that mothers stress during prenatal period of gestation provokes the firm long-lasting disturbances of fetal functional systems. The consequences of stress within early pregnance (SEP) are still researched poorly.
Aim: The aim of study was to evaluate hormonal and behaviour features in females offspring of rats with SEP.
Material and methods: For SEP creation the Wistar rat females were transferred daily into another rats association within 2nd8th days of gestation. At 12 months old the intact offspring (n=32) randomly divided into groups which were killed before (G-1) and after (G-11) functional tests. Correspondingly G-2 and G-21 were formed from stressed offspring. Corticosterone, leptin, E2, testosterone, progesterone were determined by the RIA method. Food consumption was determined by placing 200 g of fodder and weighing the residual food before and after 24 h of fasting. Perirenal, gonadal and mesenteric fat pads were removed and weighed. Data were performed statistically.
Results: It has been established that 80% animals from G-2 were heavier by 1525% than G-1. G-2 corticosterone was not distinguished from G-1 but after 24 h of fasting corticosterone was expressive increased (P<0.05, G-21 vs G-2, G-1, G-11). Offspring from G-2 had reduced leptin level after fasting and normal level through 24 h after termination of food deprivation. G-21 food consumption was increased by 1517% than in G-11 within 124 h after fasting. Weight of mesenteric fat G-21 increased by 5% through 24 h after termination of food deprivation. The stressed offspring demonstrated a high anxiety level in elevated plus-maze and decrease locomotor activity in the open field. Their testosterone level was increased by 712%, but E2 and progesterone were decreased.
Conclusion: Our findings evidence that fetus stress in the first early gestation induced negative alterations of hormonal status and behaviour in of offspring and leads to the metabolic diseases in adulthood.