Endocrine Abstracts (2010) 22 P43

Comparison of physiological and endocrine responses of rainbow trout (Oncorhynchus mykiss) after electroanesthesia and anesthesia with MS222 and clove oil

Amir Sattari1, Saed Mirzargar2, Adib Abrishamifar3 & Hoseinali Ebrahimzadeh Mosavi2


1University of Kerman, Kerman, Islamic Republic of Iran; 2University of Tehran, Tehran, Islamic Republic of Iran; 3Iran University of Science and Technology, Tehran, Islamic Republic of Iran.


This study investigated electro-anesthesia of rainbow trout (Oncorhynchus mykiss) in comparison with MS222 and clove oil by using plasma cortisol and glucose level as stress indicators. A microcontroller-based apparatus was designed and constructed to allow a programmable voltage-time pulse-width modulated (PWM) electrical wave (AC) application through 19×20 cm submersible electrodes for 91 s in a 33 cm long tank to induce loss of equilibrium and immobility with recovery after 52±27 s. Recovery after 660±102 s was observed in MS222-anesthetized fish (after induction for 720±72 s) and a recovery time of 546±102 s was observed in clove oil-anesthetized fish (after induction for 144±42 s) both are significantly longer recovery times in comparison to electro-anesthesia (P<0.001). Direct ELISA for cortisol and enzymatic and colorimetric method for glucose assessments was used at 0, 1, 6, 12 h after anesthesia in all treatments. Trend of cortisol responses during 12 h of investigation was similar in all groups. The dilatory trend of glucose changes and response derived from anesthetics and electricity and its surge at 6 h after anesthesia (P<0.05) confirmed glucose as a second order indicator of stress–responses. Electro-anesthesia is a fast, economic, eco-friendly and safe anesthetic method provides desirable trout immobility for aquaculture activities.

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