Single nucleotide polymorphism (SNP) of endothelial nitric oxide synthase (eNOS) gene (GLU298ASP VARIANT) in infertile men with asthenozoospermia
Eddi Buldreghini1, Reda Z Mahfouz2, Arianna Vignini3, Ashok Agarwal2 & Giancarlo Balercia1
Objective(s): Single nucleotide polymorphism (SNP) in endothelial nitric oxide synthase (eNOS) G894T may affect the oxidative stressresponse. Our objective was to examine G894T SNP eNOS genotype frequencies and its potential role with sperm motility in infertile men. Through this prospective controlled study in the Andrology Unit, we have enrolled infertile (n=70) and healthy (n=60) men. Sperm motion kinetics assessed by computer assisted semen analysis (CASA), and allele-specific polymerase chain reaction (PCR-RFLP) to investigate the frequency of guanine (G) thymine (T) at position −894 within exon 7 of the eNOS gene.
Finding(s): An increased frequency of the G894T eNOS (T) allele observed in asthenozoospermic patients (P=0.02). In asthenozoospermic men, homozygotes eNOS (TT) genotyping showed low percentages of rapid motile sperm (a+b) compared to wild-type eNOS (GG) (P=0.02) or heterozygotes eNOS (GT) genotyping (P=0.01). In Fertile men, wild-type eNOS showed high percentages of rapid motile sperm (a+b) compared to eNOS (TT) (P=0.03) or eNOS (GT) genotyping (P=0.04).
Conclusion(s): Our findings suggest that the T allele, encoding for aspartic acid, of the eNOS (Glu298Asp) gene may play a role with low sperm motility.