The regulatory role of miR-375 in the pancreatic β-cell secretome
Sudhir Tattikota, Thomas Rathjen, Matthias Sury, Clinton Becker, Matthias Selbach & Matthew Poy
MiR-375 regulates growth and function of the pancreatic beta cell, the source of a multitude of secreted factors into the systemic circulation which facilitate metabolic processes, most notably insulin. Here we provide mass spectrometric analysis addressing the impact of miR-375 on the pancreatic beta cell secretome. Quantitative proteomics detected over 600 proteins released in response to glucose using the murine beta cell line, MIN6. Furthermore, the secretome profiles following knockdown of miR-375 target genes revealed differential regulation of many secreted proteins highlighting the complex regulatory role of this microRNA on the secretory machinery. Many of the proteins identified here including the vitamin D binding protein, a gene previously correlated with the onset of Type 1 diabetes, have yet to be studied as a secreted factor from pancreas. This study specifically addresses the role of miR-375 in the systemic release of proteins from beta cells and provides insight into the future study of its direct targets and their role in exocytosis from this cell type.
Declaration of interest: There is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.
Funding: Declaration of Funding: European Research Council (IsletVasc 260744).