Searchable abstracts of presentations at key conferences in endocrinology
Endocrine Abstracts (2012) 29 OC3.5

ICEECE2012 Oral Communications Diabetes Clinical (6 abstracts)

Study of toll like receptor 2 and 4 expression in monocytes in type 1 diabetes mellitus with and without micro-vascular complications

S. Sedek , W. Ahmed , S. Shelbaya , R Abd El Baki & M. Abass


Shams University hospitals, Cairo, Egypt.


Background: Toll-like receptors (TLRS) are a class of proteins that play a key role in the innate immune system. They are single, membrane, non catalytic receptors which are expressed in multiple tissues predominantely cells of the innate immune system especially monocytes. So, they play a critical role in the regulation of immune function and inflammation.

Aim of our work: To study the expression of toll-like receptor 2 and 4 on monocytes in T1DM patients and its relation to microvascular complications.

Subjects and methods: This cross sectional study was conducted on 80 subjects above age of 18 years divided into Group I: 20 T1DM patients without diabetic complications. Group IIa: 9 T1DM patients with nonproliferative diabetic retinopathy (NPDR), Group IIb: 11 T1DM patients with proliferative diabetic retinopathy (PDR), Group IIIa: 7 T1DM patients with micro-albuminuria, GroupIIIb: 13 T1DM patients with macroalbuminuria, Group IV: 20 healthy subjects. They were subjected to full clinical history, thorough clinical examination, fundus examination, fasting plasma glucose level, (HbAIc), level albumin/creatinine ratio in random urine sample, measurement of TLR 2 and 4 using flowcytometry.

Results: There was highly significant difference between the studied groups as regards TLR2 and TLR4 percent (P value<0.01) being higher in T1DM with microvascular complications than in T1DM without microvascular complications than in healthy control subjects. There was also highly statistical significant positive correlation (P-value <0.01) between TLR2 and age, duration of diabetes, SBP, DBP, FPG, HbA1c and albuminuria as well as highly statistical significant positive correlation (P value<0.01) between TLR4 and age, duration of diabetes, SBP, DBP, FPG, HbA1c and albuminuria.

Conclusion: These results postulate that TLR2 and TLR4 may have a role in the microvascular complications of type 1 diabetic patients.

Declaration of interest: The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project.

Funding: This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.

Volume 29

15th International & 14th European Congress of Endocrinology

European Society of Endocrinology 

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