Searchable abstracts of presentations at key conferences in endocrinology
Endocrine Abstracts (2016) 41 EP399 | DOI: 10.1530/endoabs.41.EP399

ECE2016 Eposter Presentations Developmental endocrinology (5 abstracts)

Scanning electron microscopy of adrenal gland after laser ablation

Konstantin Kemoclidze 1 , Denis Pukhov 2 , Yuri Aleksandrov 1 & Natalya Tiumina 1


1Yaroslavl State University, Yaroslavl, Russia; 2Yaroslavl Branch at the Institute of Physics and Technology of the Russian Academy of Sciences, Yaroslavl, Russia.


Purpose: The study evaluated the effects of laser ablation of adrenal glands and results of regeneration using low-vacuum scanning electron microscopy for modeling the effects of laser ablation on pathological focus in the adrenal glands.

Methods/design: A study performed on the rats (n=19, male, Wistar line) with weighing 335.3±24.9 g. At 14 of them (control 5 intact rat) under general anaesthetic was performed local laser ablation part of left adrenal gland. For damages used surgical laser apparatus “Lami” with standard options for parenchymatous organs: point action quartz with polyamide-coated optics with a diameter of 400 μm (wavelength-1020 nm radiation power-2.5 watts). Energy exposure amounted to 70 DJ. The adrenal glands were researched without the laser exposure, immediately after it and 1 month later.

Results: Immediately after exposure occurs laser ablation crater with rough edges and melted surface. It is penetrated by equidistant pores which are footprints of blood vessels. Beneath of the surface are numerous vaporization bubbles. Around the crater the surface wrinkles and sags due to decreased ability to retain water. Through 1 month after the laser damages in the affected area is determined by a scar. Among the big bundles of collagen fibers determined by amorphous chunks of coal and caverns. Tissues with normal structure are detected near to the scar, both inside of the adrenal gland and the tissues around her. The wrinkling and the sagging are absent. The undamaged part of organ has retained the previous shape and structure without hypertrophy, the damaged part the adrenal gland reduced.

Conclusion: The nature of the regeneration processes indicates a low probability of a relapse after the destruction of a pathological focus in the adrenal after the surgical laser ablation.

Article tools

My recent searches

No recent searches.