Endocrine Abstracts

Cross-talk between adipose tissue and skeletal muscle could be mediated in part by adipokines secreted from adipose tissue. Previously, we observed that adiponectin but not leptin decreased DNA synthesis rate in proliferating porcine myoblasts in growth factor-supplemented serum-free medium (GF-SFM) after 48-h exposure (Will et al. 2012). To further elucidate the effects of adiponectin and leptin on muscle cell growth and their mode of action, this study was conducted...

The myokine irisin is secreted after cleavage of the transmembrane protein Fibronectin type III domain-containing protein five and was shown to induce a thermogenic program in white adipose tissue. This cleavage can be induced by exercise, thus linking exercise with browning of adipose tissue. We investigated the response of Fndc5/irisin as well as its inducer Peroxisome proliferator-activated receptor gamma co-activator 1α (Ppargc1a) in mice long-term selected for high t...

Patients with inactivating mutations in MCT8, known as Allan-Herndon-Dudley syndrome (AHDS), present a severe form of psychomotor retardation and, frequently, epileptic seizures of unknown etiology. These neurological symptoms are thought to arise due to an impaired transport of thyroid hormones (TH) across the blood brain barrier and/or into the neural cells. As a consequence of species-specific differences in the expression of the T4-specific organic anion transporter polype...

Thyroid hormone (TH) actions and metabolism are intracellular events that require the transport of TH across the plasma membrane. This process is facilitated by TH transporters of which the monocarboxylate transporter 8 (MCT8), encoded by the Slc16a2 gene, has been most intensively analyzed. In humans, inactivating mutations in the X-linked MCT8 gene are associated with a severe form of psychomotor retardation in combination with abnormal serum TH parameters. The clin...

Inactivating mutations in highly specific thyroid hormone (TH) transporter MCT8 result in a severe form of psychomotor retardation characterized by neurological impairments and frequent epileptic seizures of unknown etiology. These symptoms are thought to be a consequence of impaired central TH uptake across brain barriers and/or into neural cells. Mct8/Organic anion transporting polypeptide 1c1 double knockout (M/O-dKO) mice replicate characteristics of human MCT8 deficiency....

Inactivating mutations in the thyroid hormone (TH) transporter monocarboxylate transporter 8 (MCT8) result in a severe form of psychomotor retardation (known as Allan-Herndon-Dudley syndrome, AHDS) due to compromised TH access to the CNS. Consequently, TH-dependent processes both during brain development and in the adult CNS such as adult hippocampal neurogenesis are impaired. Using mice deficient in Mct8, we recently demonstrated a diminished neurogenesis in the adult hippoca...

Mice with combined deficiency in the thyroid hormone transporters Mct8 and Oatp1c1 (Mct8/Oatp1c1 dko mice) display a strongly diminished TH brain content and, consequently, a disturbed neuronal maturation and myelination as well as locomotor abnormalities while serum T3 levels are highly elevated. This phenotype can be explained by an impaired transport of T4 and T3 into the CNS in the absence of both transporters. Yet, the exact cell-specific function of Mct8 and Oatp1c1 in b...

IGF binding proteins (IGFBPs) are important biomarkers for diagnostics and treatment studies. In different physiological conditions IGFBPs are actively degraded by specific proteases. Due to the detection of intact and fragmented IGFBPs, ELISA or RIA techniques may generate biased IGFBP-data. We thus developed quantitative western ligand blotting for comprehensive monitoring of intact IGFBPs and IGFBP-profiles in biological fluids of vertebrates. The dynamic range for each IGF...

The aim of the study was to investigate the effect of different coagulants and storage conditions on the stability of ACTH in plasma using the LIAISON-ACTH-Chemiluminescence Immunoassay.Design and methods: Sequential human blood samples were collected from healthy volunteers (n=83) between 8 and 10 pm into EDTA collection tubes (Sarstedt) and EDTA plus aprotinin tubes (Vacuette, Greiner Labortechnik), respectively. After collection the blood sampl...

Impaired TH transport across brain barriers results in severe TH deficiency in Mct8/Oatp1c1 DKO mice, leading to disturbed neuronal development, myelination as well as locomotor abnormalities. Although brain-barrier associated cells (i.e. endothelial cells, astrocytes, choroid plexus epithelial cells) have been shown to express both transporters, the cell-specific function of Mct8 and Oatp1c1 has still not been defined. Here, we generated and analysed mouse mutants that lack M...