ea0026p152 | Male reproduction | ECE2011
Lukaszyk Andrzej
, Kotwicka Malgorzata
, Jankowska Anna
, Rucinski Marcin
, Kasprzak Aldona
, Sterzynska Karolina
, Ziolkowska Agnieszka
, Ruchala Marek
In this study, we attempted to demonstrate the expression of GHSR-1a receptor and possible effects of its activation in human, rat and boar spermatozoa. For demonstration of GHSR-1a immunocytochemical, immunofluorescence and western blotting techniques were applied. The effects of receptor activation was tested in a series of in vitro incubations of spermatozoa with or without addition of ghrelin and the effects were evaluated using confocal microscopy and flow cytometr...