Endocrine Abstracts

The murine ACTH receptor MC2-R is primarily expressed in the adrenal cortex and adipocytes where it binds ACTH leading to steroidogenesis and lipolysis, respectively. We have recently shown that the previously described 'adrenal' MC2-R promoter is transiently activated by PPAR gamma 2 early during differentiation of murine 3T3-L1 pre-adipocytes, declining to background levels from a peak of activity seen at day 4 following commencement of adipogenesis. This is mirrored by the ...

The peptide hormone ACTH can stimulate lipolysis and suppress leptin expression in murine adipocytes. These effects are mediated via the Melanocortin 2 Receptor (MC2-R), which is expressed when 3T3-L1 cells are induced to differentiate into adipocytes. In this study we have investigated the transcriptional activation of the MC2-R gene during adipogenesis.5' deletion analysis of the murine MC2-R promoter revealed the presence of a transcriptional enhancer...

The Melanocortin 2 Receptor (MC2R) is best known for its role in the adrenal cortex where it couples the action of ACTH to steroidogenesis, however it is also expressed by the adipocyte. We set out to characterize the up-regulation of the MC2R in the 3T3-L1 fibroblast, which can be induced to undergo adipogenesis.RT-PCR using primers specific to exons 1 and 2 amplified three bands in differentiated 3T3-L1 adipocytes which sequencing revealed to be distin...