ea0007p26 | Cytokines and growth factors | BES2004
Gavalas N
, Zarkesh-Esfahani S
, Wilkinson I
, Pradhananga S
, Beghadi C
, Baumann G
, Wu Z
, Artymiuk P
, Sayers J
, Ross R
Introduction: We have generated a truncated Growth Hormone Receptor (GHR) fused to the cell membrane by a glycosyl phosphatidylinositol anchor (GHR-GPI). The protein consists of the complete extracellular domain of the GHR, but lacks the intracellular domain, and we predict it would act as a GH antagonist. Aim: To test if partially-purified soluble GHR-GPI could re-insert into CHO cell membranes. Material and methods: Membrane preparations were made from CHO cells stably expre...