Endocrine Abstracts

TSH receptor (TSHR) expression increases >100-fold during adipogenesis and signals via CREB. Since CREB activation has been reported to be ‘necessary and sufficient to induce adipogenesis’, we investigated whether TSHR activation, a feature of most thyroid dysfunction, has a role in adipocyte biology and evaluated the contribution of Gsα and Gβγ signalling.Retroviral vectors for WT or constitutively active mutant TSHR* or ...

Adipogenesis is induced when intracellular cAMP increases and follows cell cycle arrest. During preadipocyte differentiation expression of the TSHR, which signals via cAMP and the inositol phosphate cascade, is upregulated. To investigate the role of TSHR activation in adipogenesis, we have developed an in vitro model in which constitutively active TSHR mutants (L629F, M453T) are introduced by retroviral vectors (RV).We aimed to compare the effect...

Background: Patients with an elevated aldosterone renin ratio (ARR) should proceed for a confirmatory test to diagnose primary aldosteronism (PA) before undergoing further investigations such as CT imaging and adrenal venous sampling. The recent 2016 clinical guidelines have included a new recommendation: that in the setting of spontaneous hypokalaemia, undetectable plasma renin, and plasma aldosterone concentration (PAC) >20 ng/dl, patients may not require further confirm...

Background: Styrene and ethylbenzene (S/EB), the monomers found in polystyrene (PS) and polyethylene (PE), are known to continuously leach into the environment and food products. The prevalent use of hand sanitizers containing S/EB during the COVID-19 pandemic has heightened concerns about the health implications of S/EB exposure. Notably, the impact of S/EB exposure on the risk of type 2 diabetes mellitus (T2DM) and its interaction with genetic factors in the general populati...

Objective: This systematic review aimed to assess the efficacy and safety of testosterone replacement therapy (TRT) in obese men with testosterone deficiency through the analysis of randomized controlled trials (RCTs).Materials and Methods: A comprehensive computer-based search was conducted in Cochrane Library, PubMed, Embase, Web of Science, Scopus, Open SIGLE database, China National Knowledge Infrastructure (CNKI), Wanfang Data, VIP database, and Chi...

Background and purpose: Obese men often exhibit hypoandrogenemia. Although previous research has established obesity and metabolic-related disorders as key factors contributing to decreased androgens in males, there are rare studies to comprehensively understand the correlation between sex hormones and obesity, as well as metabolic indicators, particularly in non Caucasian populations. This study aims to comprehensively investigate the relationship between sex hormones and obe...

Introduction: As a tumor-suppressor gene, armadillo repeat-containing 5 (ARMC5) gene regulates steroidogenesis and cell apoptosis in manner of second-hit model. Mutations of ARMC5 gene will disturb its normal function, leading to reduced cortisol synthesis and cell apoptosis, which is acknowledged as a frequent cause of primary bilateral macronodular adrenal hyperplasia (PBMAH). Not until another somatic ARMC5 mutation occur in germline mutation carriers, will they manifest as...

Context: Transcriptomic based characterization of signaling pathways in the adrenals of different subtypes of Cushing’s syndrome.Patients: For preliminary NGS analyses, a total of 27 adrenal samples were used. The cohort consisted of the following patient groups: Cortisol producing adenoma (CPA, n=9), primary bilateral macronodular adrenal hyperplasia (PBMAH, n=10). The adjacent normal adrenal tissue from pheochromocytoma patients ...

The cell-cell interactions that occur within the breast tumor microenvironment are critical determinants of cancer cell fate. In the face of treatment, the theory of clonal evolution dominates current thinking. Thus, individual cells may acquire a mutation(s) that provides a selection advantage, i.e., Darwinian selection acts at the single cell level. We studied the effect of antiestrogen treatment on the population remodeling ...

The cell-cell interactions that occur within the breast tumor microenvironment are critical determinants of cancer cell fate. In the face of treatment, the theory of clonal evolution dominates current thinking. Thus, individual cells may acquire a mutation(s) that provides a selection advantage, i.e., Darwinian selection acts at the single cell level. We studied the effect of antiestrogen treatment on the population remodeling ...