The relationship of insulin resistance, androgens and endothelial dysfunction in polycystic ovary syndrome ,PCOS, is controversial till now. We evaluated if ICAM-1 levels differ between PCOS and healthy women and whether this marker of the early endothelial damage is associated with the particular metabolic and/or hormonal characteristics.
After signing written informed consent, 39 PCOS women -median of age 25 yrs, BMI 24.9 and 10 age matched healthy women -median BMI 21.5, not taking hormonal therapies, cholesterol-lowering and antihypertensive drugs, underwent an euglycaemic hyperinsulinemic clamp and oral glucose tolerance test. Basal blood samples for ICAM-1, testosterone, androstendione, 17OH progesterone, DHEA S, SHBG, LH, FSH and lipid spectrum were taken in the early follicular phase of menstrual cycle. Blood pressure and anthropometric parameters were measured. Statistical evaluation: Mann Whitney test, ANCOVA and Spearmans correlations .
As expected, PCOS patients were hyperandrogenic - higher testosterone, p=0.001; DHEA S, p= 0.002; with higher LH, p= 0.007 and lower SHBG, p=0.005; with higher BMI, p=0.007 and WHR, p=0.001 in comparison with controls. The groups did not differ in the parameters of insulin sensitivity derived from the clamp. However, the higher stimulated glucose, G30 p=0.03; fasting insulin, p=0.002 and C peptide, p=0.03 as well as dyslipidaemia, lower HDL cholesterol, p=0.0007; higher triglycerides, p=0.05 in PCOS patients were detected. PCOS women had also higher ICAM-1 levels, p=0.03 even after the adjustment for BMI -ANCOVA. ICAM-1 positively correlated with basal and stimulated insulin, fasting triglycerides and with BMI, WHR, negatively with HDL cholesterol.
Conclusions: In PCOS, significantly higher levels of ICAM-1 than in healthy women were found. ICAM-1 correlated with body composition, lipids and insulin secretion, but not with insulin resistance.
The Ethical Committe of Institute of Endocrinology approved the protocol.
Supported by grant No. 301/04/1085 GACR.
04 - 06 Apr 2005
British Endocrine Societies