Searchable abstracts of presentations at key conferences in endocrinology
Endocrine Abstracts (2006) 11 P251

ECE2006 Poster Presentations Diabetes, metabolism and cardiovascular (174 abstracts)

The involvement of GH-IGF-I axis in a mouse model of type II diabetic nephropathy

Renana Eshet , Daniel Landau , Lital Wachsmann & Yael Segev


Ben Gurion University, Beer Sheva, Israel.


Type II diabetic nephropathy (DN) has become the single most common cause of end-stage renal disease in the Western world. In previous studies we have shown a significant involvement of the GH-IGF-I axis in a mouse model of type I DM. The purpose of this study was to examine the endocrine and renal changes in GH-IGF-I axis key molecules in db/db mice, a model of type II DM lacking a functional leptin receptor. Obese (D) and lean (heterozygote) (C) animals were followed, beginning at 6 weeks of age (at hyperglycemia onset), for a total of 4 weeks. Mean serum glucose levels in D animals during the study were 445±48 mg % vs 116±10 mg % in C. Serum GH levels at sacrifice were decreased in D (16±2 compared to 26±5 ng/ml in C; P<0.05). A similar decrease was seen for circulating IGF-I (237±7 vs 433±28 ng/ml in D vs C, P<0.05). Kidney weight was increased in D (169±3 vs 134±3 mg in C; P<0.05). In addition, hyperfiltration (214±18% of C), albuminuria (160±20% of C), glomerular hypertrophy (205±20%of C) and increased glomerular PAS deposition were observed in D animals. Renal IGFBP-1 protein was signifantly increased (241±39% of C) and renal IGF-I mRNA was decreased (70±7% of C) in D animals.

In conclusion, renal and glomerular hypertrophy, hyperfiltration, albuminuria and renal IGFBP-1 accumulation are seen in diabetic db/db mice already after 4 weeks of hyperglycemia, similar to finding in type I DM models. However, these renal changes are associated with low circulating IGF-I and GH levels, contrary to type I DM. This suggests that the renal complication in type II DM may be more affected by local diabetic environment.

Volume 11

8th European Congress of Endocrinology incorporating the British Endocrine Societies

European Society of Endocrinology 
British Endocrine Societies 

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