Papillary thyroid cancer (PTC) is the most common endocrine malignancy. The survival rate is excellent, however in some cases it transforms to dedifferentiated invasive tumor. Tumor cell invasion and metastasis are major causes of cancer mortality. It is a challenging task to identify critical genes controlling metastatic potential and disease recurrance. We sought molecular prognostic markers that could improve diagnostics and planning therapy.
Comparative genomic hybridisation (CGH) allows identification of changes in relative copy number of DNA sequences (gains and losses) and gives image of the primary genetic changes. We worked with a microarray based CGH method. Paraffin-embedded tissues were used. After amplification of genomic DNA with DOP-PCR the labelled PCR products were hybridized on human cDNA microarrays having 3200 gene specific samples, and scanned. Afterwards, confirmatory real-time PCR was applied.
We analysed the case of a 63-year-old man suffering in PTC. Although treated with thyreoidectomy and radioiodine ablation, we lost him in 10 months. Two possible genes were identified which might be related to the infavourable outcome and could possibly be identified already at the beginning of the disease. These two genes may be used as new prognostic markers. Our results must be confirmed in more cases, and the putative genes have to be analysed.
01 - 05 Apr 2006
European Society of Endocrinology