ISSN 1470-3947 (print) | ISSN 1479-6848 (online)

Endocrine Abstracts (2008) 16 P610

Quantitative determination of aromatase and 5-[alpha] reductase mRNA and polymorphisms in the aromatase and 5-[alpha] reductase genes in idiopathic hirsutism

Munis Dundar1, A Okay Caglayan1, Nuran Ahu Baysal2, Fatih Tanriverdi2, Yusuf Ozkul1, Kursad Unluhizarci2 & Fahrettin Kelestimur2

1Department of Genetics, Erciyes University Medical School, Kayseri, Turkey; 2Department of Endocrinology, Erciyes University Medical School, Kayseri, Turkey.

Objective: Hirsutism, affects between 5% and 10% of women during reproductive age. Hirsutism associated with normal ovulatory function and normal circulating androgen concentrations is defined as idiopathic hirsutism and little information is available regarding the molecular pathogenesis.

Aim: Significance of polymorphisms of the genes involved in androgen/oestrogen biosynthesis (SRD5A2,CYP19) and their expressions inmodulating the susceptibility to idiopathic hirsutism were studied.

Methods: Hirsutism was scored using a modified Ferriman–Gallwey scoring system. The score >8 was considered as hirsutism. All the patients had idiopathic hirsutism. We assessed the expression of the genes for type 2 (SDR5A2) 5-alpha reductase isoenzyme and aromase (CYP19) in hair follicle removed from midline subumblical region in 8 untreated idiopathic hirsute patients and 8 normal women. SDR5A2 and CYP19 expression were estimated by Real-Time-PCR using the gene of the ubiquitously expressed protein β-actin as an internal control.

Results: No differences were found in SDR5A2 expression levels between patients and normal women. CYP19 gene expression levels were lower in the patients when compared with the controls (P<0.05).

Conclusion: Decreased aromatase activity may contribute to the pathogenesis of idiopathic hirsutism.

Supported by TUBITAK (SBAG-106S170).

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