TIAR and TIA-1 are two closely related RNA-binding proteins, which possess three RNA recognition motifs (RMMs). These proteins are involved in several mechanisms of RNA metabolism, including regulation of mRNA translation. In cellular apoptosis continuously TIAR shuttled between the cytoplasm and nucleus.
The aim of this study was to estimate expression of closely related mRNA binding proteins TIAR and TIA-1 in thyroid tissues from young patients with Graves disease (GD), toxic (TNG) and non-toxic (NTNG) nodular goiter. Criteria for qualification of Graves patients: large goiter, ophthalmopathy, TRAb>5, positive titre of anti-TPO and anti-TG antibodies and concentration of TSH <0.3 more the 23 months from onset of disease. The analysis of TIAR/TIA-1 expression was performed by western blot and immunohistochemical investigation with DAB-visualization and Mayers hematoxylin staining.
Identification of mRNA binding protein TIAR in the thyroid follicular cells revealed higher expression of protein in patients with Graves disease (++) in comparison to patients with NTNG (+/0) and TNG (++/+).The detection of TIAR was presented in thyroid autoimmune disease in bands p44, p39, p36, p31 (kDa), in NTNG in band p44 and TNG in bands p44, p39, p31. TIA-1 molecule identified only in patients with Graves disease (+) in areas of intrathyroid lymphocytes.
We conclude that alteration in the expression of mRNA binding proteins TIAR and TIA-1 in Graves tissues may play role in pathogenesis of thyroid autoimmune disease.
03 - 07 May 2008
European Society of Endocrinology