Endocrine Abstracts (2008) 16 P687

Expression of RNA-binding proteins TIAR and TIA-1 in thyroid tissues from patients with immune and non-immune thyroid diseases

Artur Bossowski1, Barbara Czarnocka2, Krzysztof Bardadin3, Miroslawa Urban4, Jolanta Czerwinska5, Anna Lyczkowska6 & Jacek Dadan7


12nd Department of Children’s Diseases, Medical University in Bialystok, Bialystok, Poland; 2Department of Biochemistry and Molecular Biology, Medical Center of Postgraduate Education, Warsaw, Poland; 3Department of Pathomorphology, Medical Center of Postgraduate Education, Warsaw, Poland; 42nd Department of Children’s Diseases, Medical University in Bialystok, Bialystok, Poland; 5Department of Pathomorphology, Medical Center of Postgraduate Education, Warsaw, Poland; 6Department of Biochemistry and Molecular Biology, Medical Center of Postgraduate Education, Warsaw, Poland; 71 Department of Surgery, Medical University in Bialystok, Bialystok, Poland.


TIAR and TIA-1 are two closely related RNA-binding proteins, which possess three RNA recognition motifs (RMMs). These proteins are involved in several mechanisms of RNA metabolism, including regulation of mRNA translation. In cellular apoptosis continuously TIAR shuttled between the cytoplasm and nucleus.

The aim of this study was to estimate expression of closely related mRNA binding proteins TIAR and TIA-1 in thyroid tissues from young patients with Graves’ disease (GD), toxic (TNG) and non-toxic (NTNG) nodular goiter. Criteria for qualification of Graves’ patients: large goiter, ophthalmopathy, TRAb>5, positive titre of anti-TPO and anti-TG antibodies and concentration of TSH <0.3 more the 2–3 months from onset of disease. The analysis of TIAR/TIA-1 expression was performed by western blot and immunohistochemical investigation with DAB-visualization and Mayer’s hematoxylin staining.

Identification of mRNA binding protein TIAR in the thyroid follicular cells revealed higher expression of protein in patients with Graves’ disease (++) in comparison to patients with NTNG (+/0) and TNG (++/+).The detection of TIAR was presented in thyroid autoimmune disease in bands p44, p39, p36, p31 (kDa), in NTNG in band p44 and TNG in bands p44, p39, p31. TIA-1 molecule identified only in patients with Graves’ disease (+) in areas of intrathyroid lymphocytes.

We conclude that alteration in the expression of mRNA binding proteins TIAR and TIA-1 in Graves’ tissues may play role in pathogenesis of thyroid autoimmune disease.

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