Endocrine Abstracts (2009) 20 P465

Glucose-dependent insulinotropic polypeptide modulates inflammatory markers in adipose tissue

Özlem Gögebakan1, Martin Osterhoff1, Sandy Mosig3, Ines Middelbeck1, Natalia Rudovich2, Rita Schüler1 & AFH Pfeiffer1,2


1German Institute of Human Nutrition (DIfE), Nuthetal-Potsdam, Germany; 2Endocrinology, Diabetes and Nutrition, Charité - University Medicine Berlin, Campus Benjamin Franklin, Berlin, Germany; 3Friedrich-Schiller-University Medicine, Institute for Vascular Medicine, Jena, Germany.


Background: Glucose-dependent insulinotropic polypeptide (GIP) is a gastrointestinal hormone that is secreted in response to food intake. GIP acts on various tissues, including pancreatic ß-cells, via interaction with its G-protein-coupled receptor. Some studies in rodents suggested that GIP directly links overnutrition to obesity. GIP exerts a physiological role on lipid uptake into adipocytes.

Objectives: To analyse the effect of GIP-Infusions on changes in adipose tissue gene expression at different blood-glucose levels in obese men and also detect different biomarkers and hormone interactions caused by GIP.

Setting and participants: Seventeen healthy overweight men (BMI: 28–40 kg/m2; age: 30–65 years) with normal glucose tolerance underwent a single-blind intervention study at four different time points with euglycaemic or hyperglycaemic clamps in combination with GIP or saline infusions. Each solution was applied at physiological concentrations for 4 h. Before and after the infusions biopsies from subcutaneous adipose tissue were taken. We isolated total RNA from all fat biopsies and hybridized the RNA to Agilent Whole Human Genome Microarrays. The results were verified by RT-PCR.

Results: Using Agilent Genespring Software and the MetaCore platform we identified several genes being involved in inflammatory processes. Under euglycaemic hyperinsulinaemic clamp conditions in combinations with a GIP-Infusion we find a significant upregulation of chemokine ligand 2 (CCL-2/MCP-1), interleukin-1ß (IL-1ß) and interleukin-6.

Conclusion: GIP may play a role in inflammatory processes in adipose tissue of obese men.

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