Endocrine Abstracts (2012) 28 OC3.8

Dietary factors augment the ability of the proto-oncogene PBF to induce goitrogenesis and hyperplasia in vivo

Martin Read1, Vicki Smith1, Greg Lewy1, Gavin Ryan1, Neil Sharma1, Robert Seed1, Jim Fong1, Perkin Kwan1, Adrian Warfield2, Wendy Leadbeater1, John Watkinson2, Jayne Franklyn1, Kristien Boelaert1 & Chris McCabe1

1School of Clinical and Experimental Medicine, University of Birmingham, Birmingham, United Kingdom; 2University Hospitals Birmingham NHS Foundation Trust, Birmingham, United Kingdom.

Nodular thyroid disease is common and its pathogenesis remains poorly understood. Previously, we showed that thyroid-targeted overexpression of the proto-oncogene PTTG-Binding Factor (PBF) induced striking thyroid gland enlargement in transgenic mice (PBF-Tg)1. We have now investigated whether dietary factors influence goitrogenesis and the development of hyperplastic lesions in PBF-Tg mice. The mean weight of thyroids (5.8±0.9 mg; n=70) from 7 week old PBF-Tg mice fed on a phytoestrogen-reduced Teklad diet was significantly greater (P<0.0001) than PBF-Tg mice fed their normal RM-3 diet (3.2±0.7 mg; n=98). In contrast, there was no significant difference in the mean thyroid weight of age-matched wild-type mice fed either the Teklad (2.15±0.5 mg; n=60) or RM-3 diet (1.8±0.4 mg; n=68). This increase in thyroid weight was not related to differences in body weight (25.0±1.9g v 24.7±2.4g; P=0.5) or thyroid function, as measured by serum analysis of T3 (172.7±25.9 versus 154.1±46.9 ng/dl, P=0.45), and T4 levels (2.7±0.7 versus 3.2±1.2 μg/dl, P=0.45), between PBF-Tg mice fed either the Teklad or RM-3 diet, respectively. Analysis of mRNAs encoding growth factor receptors known to induce thyroid cell proliferation showed a significant increase in expression of PDGFR (1.6±0.3-fold, P=0.0001), FGFR2 (1.5±0.2-fold, P=0.0001), KDR (1.4±0.2-fold, P=0.003), and ERBB2 (1.4±0.2-fold, P=0.0003) in thyroids from PBF-Tg mice fed the Teklad diet compared to those on the RM-3 diet. Furthermore, focal hyperplastic lesions were detected by histological examination in thyroids from PBF-Tg mice at a younger age when fed the Teklad diet (~8 months) versus the RM-3 diet (≥12 months). Positive immunostaining with antibodies targeted to Rad51, Ung1, Trex1, and Xrcc3 also suggested extensive DNA damage within hyperplastic lesions. These results demonstrate that dietary factors can modulate the induction of goitrogenesis and hyperplasia by PBF in vivo, which has implications for understanding the relative contributions of dietary and genetic factors on the rate of nodular transformation and tumorigenesis. 1Read ML et al. (2011) Cancer Research 71(19): 6153–6164.

Declaration of interest: There is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.

Funding: No specific grant from any funding agency in the public, commercial or not-for-profit sector.