Endocrine Abstracts (2013) 32 P332 | DOI: 10.1530/endoabs.32.P332

Crystal structure of the complex between an insulin-like peptide (DILP5) and an ILP binding protein (IMP-L2) from Drosophila melanogaster

Nikolaj Kulahin1, Christopher J Watson3, Waseem Sajid1, Gerd Schluckebier2, Asser S Andersen2, Andrzej M Brzozowski3 & Pierre De Meyts1

1Novo Nordisk A/S, Gentofte, Denmark; 2Novo Nordisk A/S, Måløv, Denmark; 3University of York, York, UK.

The insulin signalling system including the insulin receptor tyrosine kinase (IRTK) is evolutionarily ancient and appears in the first multicellular organisms (Cnidarians). The Drosophila melanogaster genome contains seven genes for insulin-like peptides (ILPs) that are expressed in neurosecretory cells in a highly tissue- and stage-specific pattern, DILP1-7. There is however only one IRTK (dIR). This system is important in the regulation of metabolism, growth, reproduction and lifespan.

We reported in 2011 the crystal structure of Drosophila insulin-like peptide 5 (DILP5) (expressed in Saccharomyces cerevisiae) at 1.85Æ resolution, as well as its biological and receptor binding properties. DILP5 shares the canonical fold of the insulin peptide family and form dimers that differ from the mammalian and hagfish insulin dimers.

Drosophila melanogaster also has a circulating ILP binding protein, called imaginal morphogenesis protein-Late 2 (IMP-L2), with no equivalent in mammals. It was cloned, expressed and purified in 2000 and was shown to bind human insulin and insulin-like growth factors. We showed in 2011 that it also binds DILP5. The knockout of IMP-L2 is embryonic lethal.

We now report the solution of the crystal structure of IMP-L2 in the free form as well as bound to DILP5. Recombinant IMP-L2 was expressed in a Baculovirus expression system, purified and crystallized with selenium as heavy atom.

IMP-L2 showed as predicted from the sequence a bilobed structure with two IgG beta sheet modules (3 strands for IgGI and 4 for IgGII), folded together into a ‘baseball glove’. The structure is very different from the known partial structure of IGFBP5 which is mostly alpha-helical.

The complex of IMPL-2 with DILP5 showed a multimeric structure with two DILP5 molecules bound into the grooves between the beta sheets of two distinct IMP-L2s in a symmetrical tetrameric IMPL-2. The structural differences between the free and bound forms will be discussed.

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