Searchable abstracts of presentations at key conferences in endocrinology
Previous issue | Volume 35 | ECE2014 | Next issue

16th European Congress of Endocrinology

Poster Presentations

Nuclear receptors and signal transduction

ea0035p736 | Nuclear receptors and signal transduction | ECE2014

Formation of progesterone receptor-NF-κB complex is required for progesterone-induced NF-κB nuclear translocation and binding onto the p53 promoter

Hsu Sung-Po , Yang Ho-Ching , Kuo Chun-Ting , Wen Heng-Ching , Chen Li-Ching , Huo Yen-Nien , Lee Wen-Sen

We previously showed that progesterone (P4) regulates p53 expression and cell cycle progress in human umbilical venous endothelial cells (HUVEC) through progesterone receptor (PR) activation of extra-nuclear signaling pathways. Here, we showed that P4 activated the PR and hence increased the formation of progesterone receptor A-(PR-A)-NF-κB complex in both the cytosol and the nucleus. Chromatin immunoprecipitation demonstrated an interaction between PR and the NF-κB ...

ea0035p737 | Nuclear receptors and signal transduction | ECE2014

The effect of hormone-induced PtdIns (4, 5) P2 depletion on endocytosis suggests the importance of local regulation of inositol lipid signalling

Toth Daniel J , Toth Jozsef T , Tallosy Bernadett , Hunyady Laszlo , Varnai Peter

Receptor endocytosis plays an important role in regulating the responsiveness of cells to specific hormones. Phosphatidylinositol 4, 5-bisphosphate (PtdIns(4, 5)P2) has been shown to be critical for many endocytic processes including the internalization of G protein-coupled receptors (GPCRs). We have previously shown that depletion of PtdIns(4, 5) P2 by the chemically induced plasma membrane recruitment of a 5-phosphatase domain prevents the internalizati...

ea0035p738 | Nuclear receptors and signal transduction | ECE2014

Thyroid receptors antagonizes TGFβ actions in vivo and in culture cells

Merino Elvira Alonso , Martin Orozco Rosa M , Llorente Lidia Ruiz , Fanjul Rodriguez Luisa Fernanda , Martinez-Iglesias Olaia , Velasco-Martin Juan Pedro , Regadera Javier , Aranda Ana

Transforming growth factor beta (TGFβ), which plays a key role in cancer and fibrotic disorders, mediates its actions mainly through activation of Smad transcription factors, which bind to Smad Binding Elements (SBEs) in target genes. We have previously observed that the thyroid hormone T3 blocks transactivation of SBE-containing reporter plasmids by TGFβ, and represses transcription of endogenous TGFβ target genes. We have now analysed the thyroid ho...

ea0035p739 | Nuclear receptors and signal transduction | ECE2014

Increased irisin abundance in muscle and blood circulation after treadmill exercise in mice

Brenmoehl Julia , Albrecht Elke , Komolka Katrin , Schering Lisa , Langhammer Martina , Hoeflich Andreas , Maak Steffen

The myokine irisin is secreted after cleavage of the transmembrane protein Fibronectin type III domain-containing protein five and was shown to induce a thermogenic program in white adipose tissue. This cleavage can be induced by exercise, thus linking exercise with browning of adipose tissue. We investigated the response of Fndc5/irisin as well as its inducer Peroxisome proliferator-activated receptor gamma co-activator 1α (Ppargc1a) in mice long-term selected for high t...