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16th European Congress of Endocrinology

Poster Presentations

Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>)

ea0035p826 | Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2014

Copeptin concentrations increase during glucagon stimulation test: possible role of copeptin in assessment of anterior pituitary function

Lewandowski Krzysztof , Lewinski Andrzej , Skowronska-Jozwiak Elzbieta , Stasiak Magdalena , Horzelski Wojciech , Brabant Georg

Background: Copeptin, the C-terminal part of the arginine vasopressin (AVP) precursor, is a 39-amino acid peptide stoichiometrically secreted with AVP in 1:1 ratio from the posterior pituitary. In contrast to AVP, copeptin remains stable ex vivo for several days in serum or plasma. Here, we investigated the use of the copeptin assay in the diagnostic workup of patients with suspected anterior pituitary dysfunction.Patients and methods: We measur...

ea0035p827 | Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2014

Effect of ubiquitin/proteosome inhibition on acth secretion by rat corticotropes

Sesta Antonella , Cassarino Maria Francesca , Cavagnini Francesco , Giraldi Francesca Pecori

ACTH is the product of proteolytic processing of its precursor, proopiomelanocortin (POMC) and, in turn, is subject to proteolytic degradation. Regulation of proteolysis is crucial to several intracellular processes, including protein activation and inactivation, and thus contributes to active peptide concentrations. The proteosome 26S/ubiquitin system (UPS) is the main, non-lisosomial intracellular proteolytic pathway and alterations to this system have been observed in both ...

ea0035p828 | Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2014

Different expression of prolactin receptor gene in rat duodenum during physiological and medicamentous hyperprolactinemia

Radojkovic Danijela , Pesic Milica , Antic Slobodan , Radenkovic Sasa , Kostic Sonja , Curkovic Sanja , Vukelic Marija , Jevtovic-Stoimenov Tatjana

Introduction: During pregnancy (state with physiological hyperprolactinemia-HP) mothers require larger calcium amount for fetal growth. It has been shown that PRL stimulate intestinal calcium absorption in physiological HP, which can protect mother’s skeletal system from further mineral density loss. On the other hand, medicamentous-related hyperprolactinemia is more frequently associated with reduced BMD and increased fracture risk. We conducted the experimental study to...

ea0035p829 | Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2014

Prothymosin alpha and Ki-67 expression in pituitary adenomas

Golkowski Filip , Wierzbicka-Tutka Iga , Sokolowski Grzegorz , Baldys-Waligorska Agata , Adamek Dariusz

Introduction: As observed clinically, the development of pituitary tumours and the probability of their recurrence after neurosurgery tend to be unpredictable. The aim of this work was to evaluate Ki-67 and nuclear and plasmatic prothymosin alpha indices as potential pathological markers to predict the aggressiveness of pituitary adenomas.Material and methods: Ki-67 and prothymosin alpha indices were determined by immunochemistry in specimens excised fro...

ea0035p830 | Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2014

Dopamine receptor type 2 (dopamine receptor type 2) inhibits non-functioning human pituitary tumor-derived cell line HP75 migration through ROCK-mediated cofilin inactivation

Treppiedi Donatella , Peverelli Erika , Giardino Elena , Spada Anna , Mantovani Giovanna

Neurosurgery is the treatment of choice of non-functioning pituitary tumors (NFPAs), but its success is strongly affected by local invasion. Medical therapy is still under debate, although the use of cabergoline results in tumor shrinkage in a subset of patients. We recently demonstrated that dopamine receptor DRD2 agonist BIM53097 exerts cytostatic and cytotoxic effects in cultured cells from NFPAs, but no data are present in literature about DRD2 mediated effects on pituitar...

ea0035p831 | Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2014

Functional characterization of a new deletion in CDKN1B 5′-UTR region

Ruvo Mauro Di , Tagliati Federico , Gentilin Erica , Gagliano Teresa , Pellegata Natalia , Benfini Katiuscia , Uberti Ettore degli , Zatelli Maria Chiara

Introduction: CDKN1B gene, which encodes a cyclin-dependent kinase (Cdk) inhibitor, regulates the progression throughout G1 to S cell cycle progression. CDKN1B loss-of-function germinal mutations cause the multiple endocrine neoplasia type 4 syndrome (MEN4).Objective: The aim of the study is the functional characterization of a new 4 bp deletion in CDKN1B 5′-UTR region, identified in an acromegalic patient.Materials and metho...

ea0035p832 | Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2014

Correlation of coexpression of pituitary genes in QPCR and microarray study in pituitary adenomas

Zebracka-Gala Jadwiga , Larysz Dawid , Pfeifer Aleksandra , Rudnik Adam , Krajewska Jolanta , Hasse-Lazar Kornelia , Kowalska Malgorzata , Bazowski Piotr , Jarzab Barbara

Introduction: Mechanism of pathogenesis of pituitary adenomas is still unknown. Gene expression differences in pituitary cells of different origin are not extensively described. Identification of genes specific for pituitary adenomas should enable better understanding of differences in their response to therapy, especially to radiotherapy.Aim: The aim of our study was to evaluate the correlation of coexpression of distinct pituitary adenoma genes based o...

ea0035p833 | Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2014

Germline aryl hydrocarbon receptor interacting protein (AIP) gene mutations in patients with apparently sporadic pituitary macroadenomas (PMA): initial results

Skalniak Anna , Trofimiuk-Muldner Malgorzata , Pietkowski Jakub , Sokolowski Grzegorz , Hubalewska-Dydejczyk Alicja

Germline AIP gene mutations have been linked with familial isolated pituitary adenomas (FIPA). Inactivating mutations of AIP have also been reported in seemingly sporadic pituitary adenomas, particularly of early onset, aggressive, and GH secreting.Aim: To assess the frequency and type of germline AIP gene mutations in patients with apparently sporadic PMAs.Material: The study included 31 consecutive patients with pituitary macroad...

ea0035p834 | Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2014

Expression of peroxisome-proliferator activated receptor α in pituitary tumours

Rotondi Sandra , Modarelli Alessio , Rostomyan Lilya , Oliva Maria Antonietta , Esposito Vincenzo , Ventura Luca , Arcella Antonietta , Alesse Edoardo , Beckers Albert , Jaffrain-Rea Marielise

Peroxisome-proliferator activated receptors (PPARs) are involved in a number of neoplasia. PPARα (PPARα) is a partner of the aryl hydrocarbon receptor interacting protein (AIP), which is involved in the pathogenesis of pituitary adenomas (PA). We wished to investigate the potential expression and biological significance of PPARα in PA, especially in GH/PRL-secreting tumours.Material and methods: A large series of PA was collected (n</e...

ea0035p835 | Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2014

AIP expression in non-functioning pituitary adenomas is strongly associated with the gonadotroph phenotype but not with tumour aggressiveness

Rotondi Sandra , Oliva Maria Antonietta , Esposito Vincenzo , Ventura Luca , Giangaspero Felice , Alesse Edoardo , Jaffrain-Rea Marielise

The aryl hydrocarbon receptor interacting protein gene has been mainly involved in the pathogenesis of GH/PRL-secreting pituitary adenomas (PA). We wished to study the significance of AIP expression in clinically non-functioning PA (NFPA).Material and methods: 48 NFPA -27 gonadotroph (GnPA), 21 null cell (ncPA) – were studied for AIP, βFSH and cyclin D1 expression by real-time RT-PCR (qRT), compared with four normal post-mortem pituitaries (NP)...

ea0035p836 | Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2014

Survivin expression in invasive pituitary gland adenomas with a diameter exceeding 20 mm

Waligorska-Stachura Joanna , Sawicka-Gutaj Nadia , Andrusiewicz Miroslaw , Ruchala Marek

Introduction: Survivin is a multifunctional protein and acts as an inhibitor of apoptosis. Its expression occurs in nearly all human cancers. In some tumors survivin expression correlates with the malignant behavior and diminished response to cytotoxic therapy. Data concerning survivin expression in invasive pituitary gland adenomas are contradictory.Patients and methods: Survivin expression was assessed in 38 invasive pituitary gland adenomas (31 non-fu...