Aim to study the particularities changes of organometric parameters of rats adrenal glands after 60-day administration of sodium benzoate and justify possible correction of identified changes by application of mexidol.
The study was conducted on 90 mature male rats with an initial weight of 200210 g, which were divided into three groups: 1st group control animals, 2nd group rats that within 60 days daily intragastrically injected 1 ml of sodium benzoate at a dosage of 500 mg/kg, 3rd group rats that within 60 days daily intragastrically injected 1 ml of sodium benzoate at a dosage of 500 mg/kg and subcutaneously 5% solution of mexidol at a dosage 50 mg/kg. Animals were withdrawn from the experiment on 3 day after the end administration of the sodium benzoate. Using digital calipers, accurate to 0.05 mm measured length, width and thickness of the left and right adrenal glands.
In the animals of 2nd group length of right adrenal gland decreased, as compared with the 1st group, from 3.46±0.04 to 3.00±0.10 mm (13.29%, P<0.05), length of the left from 3.30±0.08 to 2.44±0.02 mm (26.06%, P<0.05), and the thickness of the left adrenal gland from 2.42±0.10 to 2.14±0.07 mm (11.57%, P<0.05). In animals of 3rd group length of the right adrenal gland increased, compared with the parameters of the 2nd group, from 3.00±0.10 to 3.96±0.18 mm (32.00%, P<0.05), length of the left from 2.44±0.02 to 3.23±0.15 mm (32.37%, P<0.05), the width of the right from 3.72±0.07 to 4.50±0.08 mm (20.97%, P<0.05), the thickness of the right from 2.40±0.11 to 2.98±0.12 mm (24.17%, P<0.05), and the left adrenal gland from 2.14±0.07 to 2.86±0.14 mm (33.64%, P<0.05).
Thus, the 60-day daily administration of sodium benzoate mature animals accompanied by a decrease of the linear dimensions of the adrenal glands and the application of mexidol a at dosage of 50 mg/kg smoothes revealed changes.
03 - 07 May 2014
European Society of Endocrinology