Endocrine Abstracts

Context: Successful used of temozolomide (TMZ) treatment has been described in 40–50% of aggressive pituitary tumour (PT) or rare pituitary carcinoma. These results are based on 50 case-reports and small series, data on long-term follow-up being rare.Objectives: To describe initial results and long-term follow-up of a large French cohort of patients presenting PT treated with temozolomide.Design: Members of the French Society ...

The genetic instability, i.e. loss of heterozygosity (LOH) and microsatellite instability (MSI) are frequent molecular events in thyroid tumour aetiopathogenesis. They were found in several chromosomal critical areas, including 3p12–p21.2, 3p24.2–p25.3, 7q21.1–q31.2, 10q22–24, and 15q11–q13, with loci of oncogenes and tumour suppressor genes.Aim of the study: Evaluation of usefulness of LOH/MSI as diagnostic/prognostic biomarker ...

Cancer cells show different metabolic requirements than normal cells, being the high rate of glucose uptake one of the most important aspects. An increase in glucose uptake has been associated mainly with GLUT1 overexpression but may also involve other transporters including GLUT4, whose presence in prostate cancer cells was recently discovered in our laboratory. Moreover, androgens stimulate anabolic synthesis and increase glucose uptake. Despite this, the regulation of GLUT ...

Introduction: Type 1 iodothyronine deiodinase (DIO1) is one of the three enzymes regulating bioavailability of thyroid hormones. In contrast to DIO2 and DIO3, the specific cellular role of DIO1 remains controversial. Our previous studies showed that DIO1 expression in clear cell renal cell carcinoma (ccRCC) is decreased, followed by lowered intracellular T3 concentration. In this study we explored how re-introduction of DIO1 in ccRCC cells affects their mig...

FSH receptor (FSHR) expression has been shown in gonadal tumours, as well as in endothelial tumour vessel cells of various cancers. FSHR, due to its transmembrane localization could be a good candidate for receptor-mediated targeted cancer therapy. In recent years, a number of membrane disrupting lytic peptides have been used for receptor-based cancer therapy. In the present study, we characterised the specificity and cytotoxicity of a lytic peptide Phor21 conjugated to three ...

Though there is no evidence of a constitutive nuclear accumulation of β-catenin, the Wnt/β-catenin pathway might be deregulated in parathyroid tumours. We investigated unphosphorylated active β-catenin distribution by western blot in 16 typical parathyroid adenomas (PAds): β-catenin accumulation in the nuclear protein fractions varied from the levels detected in Caco-2 cells with constitutively active Wnt signalling (three PAds) to the levels measured in HE...

Introduction: Prostate cancer (PC) is dependent on androgen receptor (AR) activation by its canonical ligands testosterone and 5α-dihydrotestosterone (DHT). Intratumoural androgens persisting after castration give rise to castration-resistant PC (CRPC). These intraprostatic androgen levels are hypothesized to result from either adrenal androgen conversion or intratumoural de novo DHT synthesis through the classic or alternative pathways. Quantifying the steroid f...