ECE2015 Eposter Presentations Calcium and Vitamin D metabolism (96 abstracts)
Development of an ELISA for the measurement of free 25OH vitamin D
Nicolas Heureux 1 , Michel Anciaux 1 , Martin Poncelet 1 , Fabienne Mathieu 1 , Leon Swinkels 2 , Tonnie Huijs 2 , Saskia Boerrigter 2 , Ernst Lindhout 2 , Gregg Mayer 2 & Mike Martens 2
1DIAsource Immunoassays, Louvain‐la‐Neuve, Belgium; 2Future Diagnostics BV, Wijchen, The Netherlands.
The objective of this project was to develop and characterise a simple immunoassay for the quantification of free 25-hydroxyvitamin D (25OH Vit D). Recent studies suggest that the concentration and genotype of vitamin D binding protein (DBP) are important factors that determine the bioavailability of 25OH Vit D in blood. It has been suggested that measurement of free, non-protein bound 25OH Vit D in serum, may provide more relevant diagnostic information than total 25OH Vit D, for instance in chronic kidney disease, bladder cancer and pancreatic cancer, or in haemodialysis patients. A two-step enzyme-linked immunosorbent assay (ELISA) was developed for the quantification of free 25OH Vit D assay, using a highly specific anti-25OH Vit D monoclonal antibody. The assay was calibrated against a symmetric dialysis method. The calibrator range was 0–35 pg/ml. The LoB was 1.1 pg/ml; the LoD was 1.7 pg/ml. Total assay precision was 5.7% at 6.3 pg/ml, 3.8% at 15.9 pg/ml and 4.8% at 24.8 pg/ml. The assay was recently compared with calculated free 25OH Vit D in clinical populations (J Schwartz J. Clin. Endocrinol. Metab. 2014). An assay was developed that reproducibly determines the level of free 25OH Vit D in serum. The assay can be used as a valuable tool in studies to establish the clinical relevance of free 25OH Vit D.
Volume 37
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more