Searchable abstracts of presentations at key conferences in endocrinology
Endocrine Abstracts (2015) 37 EP653 | DOI: 10.1530/endoabs.37.EP653

ECE2015 Eposter Presentations Pituitary: basic and neuroendocrinology (62 abstracts)

The NAD- and NADP-dependent blood lymphocytes dehydrogenases level and their interaction with GH/IGF1 in active acromegaly

Margarita A Dudina 1, , Sergey A Dogadin 1, & Andrey A Sacvhenko 2


1Krasnoyarsk State Medical University named after Prof. V. F. Voino-Yasenetsky, Krasnoyarsk, Russia; 2Siberian Division, Institute for Medical Problems of the North, Russian Academy of Medical Sciences, Krasnoyarsk, Russia; 3Krasnoyarsk Regional Clinical Hospital, Krasnoyarsk, Russia.


Somatotropin (GH) and IGF1 realise their influence on the system of intracellular metabolism and a number of important biochemical lymphocytes reactions through the receptor apparatus. It is allows to use the peripheral blood lymphocytes as the investigations object of intracellular metabolism disorders in acromegaly.

Aim: To study the NAD- and NADP-dependent dehydrogenases activity in blood lymphocytes in active acromegaly patients.

Methods: The activity of NAD(P)-dependent dehydrogenases in blood lymphocytes was studied in a group of 88 patients (35 men and 53 women) with active acromegaly, mean age 51.0±12.5 years. The NAD(P)-dependent dehydrogenases activity was determined by biochemiluminescence method. The concentrations of GH and IGF1 were measured by ELISA.

Results: Studying the activity of mitochondrial NAD(P)-dependent dehydrogenases found a decrease in all NAD-dependent oxidoreductase: NADIDH, NADGDH, and MDH (P<0.01), which allows to state the low level flow in the tricarboxylic acid cycle. In active acromegaly were revealed the decreasing activity of all studied oxidoreductases: glucose-6-phosphate dehydrogenase (P<0.01), NAD–lactate dehydrogenase (LDH) (P<0.001), NADH–LDH (P<0.001), NAD–malate dehydrogenase (MDH) (P<0.001), NADH–MDH (P<0.001), NADP–MDH (P<0.001), NAD–glutamate dehydrogenases (GDH) and NADH–GDH (P<0.001), NADP–GDH and NADPH–GDH (P<0.001), NAD–isocitrate dehydrogenases (IDH) and NADP–IDH (P<0.01 and P<0.001 respectively), and glutathione reductase (P<0.001). Our data observed that decreasing activity of NADP–GDH positively correlated with the basal GH level (r=+0.23, P=0.04) and NADP–MDH activity with IGF1 level (r=+0.30, P=0.008). The low NADH–MDH activity negatively correlated to the basal GH concentration (P=−0.23, P=0.04).

Conclusion: The chronic excess of GH and IGF1 in acromegaly causes a significant depletion of metabolic lymphocytes reserves. The main indicators of functional lymphocytes impairment in acromegaly are: the reduction of intermediates formation for the reactions of macromolecular synthesis and aerobic processes, the low intensity of glycolysis, nitrogen metabolism and inhibition of glutathione complex activity.

Article tools

My recent searches

No recent searches.