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Endocrine Abstracts (2016) 41 EP960 | DOI: 10.1530/endoabs.41.EP960

ECE2016 Eposter Presentations Steroid metabolism + action (13 abstracts)

17β-estradiol or triclosan-induced epithelial-mesenchymal transition and migration of MCF-7 breast cancer cells were reversed by kaempferol, a phytoestrogen

Geum-A Lee , Kyung-A Hwang & Kyung-Chul Choi


Laboratory of Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.


As a phytoestrogen, kaempferol plays a chemopreventive role inhibiting cancer formation and progression. In this study, chemopreventive activity of kaempferol was examined by measuring its effect on growth, epithelial-mesenchymal transition (EMT), and migration of MCF-7 breast cancer cells increased by 17β-estradiol (E2, a positive control) or triclosan (TCS), an endocrine-disrupting chemical (EDC). As an EDC, TCS is known to interfere estrogen receptor (ER) dependent pathway in MCF-7 cells expressing ERs. In MTT assay, TCS (10−4–10−7 M) or E2 (10−9 M) induced cell growth of MCF-7 cells, which was reversed to a control level by co-treatment of ICI 182,780 (10−8 M), an ER antagonist, or kaempferol (50 uM). In a wound-healing scratch assay, TCS enhanced migration of MCF-7 cells like E2, but co-treatment of kaempferol or ICI 182,720 decreased the migration ability of MCF-7 cells to a control level. In RT-PCR and western blot assay, we examined the effect of TCS and DIM on mRNA and protein expression of EMT-related markers such as E-cadherin, N-cadherin, snail and slug. TCS induced the increased expression of EMT promoting markers such as N-cadherin, snail and slug but down-regulated the expression of E-cadherin, an epithelial marker inhibiting EMT process. On the contrary, kaempferol was shown to reverse the expression pattern of EMT-related markers induced by TCS or E2. In conclusion, kaempferol effectively suppressed growth, EMT, and migration ability of MCF-7 breast cancer cells increased by E2 and TCS.

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