Endocrine Abstracts (2016) 42 IL5 | DOI: 10.1530/endoabs.42.IL5

Droplet Digital PCR: Resolving difficult challenges in nucleic acid detection and quantitation

Francisco Bizouarn



The Polymerase Chain Reaction is going through a renaissance with the arrival of new Digital PCR instrumentation. Amongst these, Droplet Digital PCR is embraced by the research community as an affordable, high resolution quantitative and detection analysis solution for nucleic acids. This presentation will cover the concept of digital PCR, absolute and standard free quantitation of nucleic acid targets as well as applications; CNV analysis, Mutation Abundance, Single cell expression analysis and genome editing event screening, that take advantage of high resolution quantitation and partitioning.

DOI 10.1530/endoabs.42.IL5

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