Endocrine Abstracts (2017) 50 P188 | DOI: 10.1530/endoabs.50.P188

The state of glutathione system in patients with type 2 diabetes

Volha Shyshko1, Tatjana Mokhort1, Natalia Tsapaeva1 & Inna Buko2

1Belarusian State Medical University, Minsk, Belarus; 2Republican Centre for Hygiene, Epidemiology and Public Health, Minsk, Belarus.

Introduction: Glutathione system prevents oxidative stress development in erythrocites. When this system becomes depressed it predisposes a cell to apoptosis.

Aim: To study glutathione system status in patients with diabetes

Materials and methods: Included patients were divided into four groups: group 1–41 almost healthy person (control group), group 2–41 patients with type 2 diabetes (T2D), group 3–40 patients with T2D and coronary heart disease (CHD) and group 4–88 patients with CHD. Activities of glutathione peroxidase (GP, mmol/min) and glutathione reductase (GR, mmol/min) and concentrations of reduced glutathione (GSH, mmol/l), oxidized glutathione (GSSG, mmol/l) and redox-status (GSH/GSSG) were measured. A1c level was <7.5%, patients with anemia and acute cardiovascular diseases were excluded.

Results: Activities of GP, GR and concentrations of GSH, GSSG and redox-status of glutathione system presented in Table 1. GP activity was increased statistically significant in patients with T2D and T2D and CHD (P<0.05) and GR activity was increased in patients with CHD (P<0.05) compared to control group. Concentrations of GSH was decreased in groups 2 and 3 (P<0.05) conpared to almost healthy persons. But concentration of GSSG was significantly higher only in patients with T2D and CHD. Also CHD was associated with depression of redox-status with maximum decrease when T2D is associated with CHD.

Table 1 Activities of GP, GR and concentrations of GSH, GSSG and redox-status of glutathione system (Me (LQ;UQ)
FindingsGroup 1Group 2Group 3Group 4
GP44.97 (36.96;58.70)51.01 (31,36;67,52)60.13 (50,25;70,12)45.58 (36.73;52.32)
GR0.89 (0.69;1.10)0.84 (0.76;1.18)1.12 (0,81;1,28)1.47 (1.32;1.88)
GSH2.14 (1.98;4.19)2.08 (2.00;2.92)1.34 (0.61;1.41)1,47 (1.32;1.88)
GSSG0,33 (0.31;0.36)0,31 (0.30;0.32)0.40 (0.36;0.40)0.36 (0.33;0.38)
GSH/ GSSG6.48 (6.38;11.64)6.70 (6.67;9.12)3.35 (1.69;3.53)4.08 (4.00;4.95)

Conclusion: T2D was associated with increased activity of GP. Decreased concentration of GSSG, increased concentration of GSH and depressed redox-status of glutathione system can be used as additional markers for early prognosis of atherosclerosis development in patients with T2D.

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