Endocrine Abstracts

Introduction: Vitamin D deficiency is a global problem worldwide.

Purpose of the study: To investigate the incidence of vitamin D deficiency and genetic sensitivity to it among patients in the polyclinic.

Materials and methods: A retrospective study was conducted, 6034 medical charts were analyzed, of those who were tested for vitamin D, the study included 567 patients (374 women, 193 men, women average age 40.7±14.1 years, men – 41.3±12.7 years). The following were analyzed: total calcium, creatinine, LDL, parathyroid hormone, urine protein. A genetic test was performed on the sensitivity of the receptors to vitamin D by sequencing HiSeq2000 on DNA chips (Illumina). An interpretation was made of the genetic characteristics of sensitivity to vitamin D: a predisposition to a decrease in receptor sensitivity; with a predisposition to high receptor sensitivity. The statistical analysis of the data was carried out with the help of the package of applied programs Statistica 10.0.1011.0. The critical level of reliability of the null hypothesis was assumed to be 0.05.

Results: Deficiency of vitamin D (25%, mean – 16.2±3.4 ng/ml), moderate vitamin D deficiency (37%, mean – 24.9±2.5 ng/ml), normal vitamin D concentration (38%, mean – 39.7±9.6 ng/ml). A weak negative correlation was found between vitamin D and LDL (P<0.05, r=-0.16). In the pairwise comparison (t-tests), significant differences in the seasons of the year were revealed for vitamin D levels: in winter groups (mean – 24.9±9.97 ng/ml) and summer (mean – 31.2±13.42 ng/ml), as well as in the autumn groups (mean – 29.8±10.18 ng/ml) and spring (mean – 26.9±10.9 ng/ml) (P=0.00003 and P=0.01 respectively). In the case of a pair comparison (t-tests), significant differences were detected by the level of total calcium and significantly differ in groups with stage 1 CKD (mean – 2.33±0.1 mmol/l) from stage 2 CKD (mean – 2.38±0.09 mmol/l) and from stage 3 CKD (mean – 2.53±0.13 mmol/l) (P=0.017 and P=0.0009 respectively). In a pair comparison (t-test), significant differences in the level of vitamin D in groups with a predisposition to a decrease in receptor sensitivity (mean – 34.6±10.54 ng/ml) and a predisposition to high sensitivity of receptors (mean – 28.3±11.42 ng/ml) (P=0.015).

Conclusions: The concentration of vitamin D by the genetic test is related to the sensitivity of the receptors.