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Endocrine Abstracts (2018) 56 P351 | DOI: 10.1530/endoabs.56.P351

Department of endocrinology and diabetology Medical clinic 1, Frankfurt am Main, Germany.


Type 2 diabetes (T2D) patients have a high cardiovascular risk due to vascular inflammation and dyslipidaemia. Furthermore vitamin D (VD) deficiency is highly prevalent. Our aim was to elucidate the role of fatty acid receptors in inflammatory pathways and their regulation by VD. We therefore examined the VD effect on gene expression of Arachidonate 5-lipoxygenase (ALOX-5) and Sphingosine-1-phospate receptors (S1PR1 and S1PR2) in primary isolated monocytes of T2D patients and healthy controls (HC). CD14+ monocytes (Mo) were isolated from 20 healthy controls (HC) and 20 T2D patients and were treated for 24 h with / without 10−8 M calcitriol. Interleukin-1β as an inflammatory stimulant served as IL-1βcontrol-cells. CD14, ALOX-5, S1PR1 and S1PR2 mRNA expression levels were measured by TaqMan analyses. 18s rRNA served as a house keeping gene. Gene expressions were defined as 2-[Ct (target)-Ct (18srRNA)]. Calcitriol treatment significantly increased the CD14 gene expression in both HC (CD14 calcitriol: 5059 vs. 2104; P=0.05) and T2D (CD14calcitriol: 12176 vs. 6712; P=0.001) compared to the IL-1βcontrol-cells. The CD14 gene expression was noticeable increased in T2D patients compared to HC (P=0.002). Moreover ALOX-5 mRNA-levels were also increased by calcitriol compared to the IL-1βcontrol-cells in HC (ALOX-5calcitriol: 1686 vs. 1004; P=0.02) and in T2D (ALOX-5calcitriol: 2744 vs. 1372; P=9.1×10-5). By comparing HC vs. T2D, it stands out that ALOX-5 mRNA-levels were higher in T2D patients compared to HC (P=0.01). Furthermore, the mRNA levels of S1PR2 were significantly reduced in both HC (S1PR2calcitriol: 5.75 vs. 25; P=10−6) and T2D (S1PR2calcitriol: 5.96 vs. 36; P=0.001) compared to the IL-1βcontrol-cells. Interestingly, T2D patients mRNA levels of S1PR2 of IL-1βcontrol-cells compared to HC were significantly higher (P=0.03). Calcitriol treatment did not change the mRNA levels of S1PR2 by comparing HC vs. T2D. No significant changes of the S1PR1 mRNA expression were observed in calcitriol treated Mo from HC and T2D patients. In vitro calcitriol increased the CD14 and ALOX-5 gene expression in HC and T2D-patients.However, the increase of CD14 and ALOX-5 gene expression in T2D appears to be disease specific. Further calcitriol had no impact on S1PR1 -but on S1PR2 mRNA expression. Although calcitriol reduced the gene expression of S1PR2 both in T2D-patients and HC this was not discriminatory. These results provide novel insights into potential anti-inflammatory mechanisms of VD in type 2 diabetes.

Volume 56

20th European Congress of Endocrinology

Barcelona, Spain
19 May 2018 - 22 May 2018

European Society of Endocrinology 

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