Impact of neurodegeneration on GLUT4 activity in experimental dm rats brain hyppocampus

Mukhammadjon Mustafakulov; Tokhir Ishanhodjaev; Gulnora Artykbaeva; Talat Saatov; Zulaykho Shamansurova; Ashok Srivastava

doi:10.1530/endoabs.110.EP980

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Endocrine Abstracts (2025) 110 EP980 | DOI: 10.1530/endoabs.110.EP980

ECEESPE2025 ePoster Presentations Metabolism, Nutrition and Obesity (164 abstracts)

Impact of neurodegeneration on GLUT4 activity in experimental dm rats brain hyppocampus

Mukhammadjon Mustafakulov ¹ , Tokhir Ishanhodjaev ¹ , Gulnora Artykbaeva ¹ , Talat Saatov ¹ , Zulaykho Shamansurova ^1,2 & Ashok Srivastava ³

Author affiliations

¹Institute of Biophysics and Biochemistry at the National University of Uzbekistan, Metabolomics, Tashkent, Uzbekistan; ²Central Asian University, Endocrinology, School of Medicine, Tashkent, Uzbekistan; ³University of Montreal, Montreal, Canada

JOINT2167

Introduction: Cell membrane function abnormalities were seen in type 2 Diabetes Mellitus (DM) and leads to abnormal insulin signaling transduction followed decreased activity of GLUT4. Neurodegeneration also affects brain function by affecting GLUT4 activity. We studied GLUT4 activity in rats brain hyppocampus in rats with experimental streptozotocine induced DM in compare with additional neurodegeneration state.

Material and Methods: Adult 12 week aged rats were divided into 3 groups, experimental streptozotocine induced DM fed standard chow (DM) and fed with high fat diet and neurotoxin (DM+N), also control group without DM. Experimental DM development were evaluated in blood samples taken from tail vien by glucometer. After 12 weeks experiments rats were sacrificed and brain samples were flash frozen for further measure of GLUT4 acitity in extracted hyppocampus by ELISA.

Results: DM rats blood glucose level were increased 2.1 times in compare with control rats and confirm streptozotocin induced DM model. Blood glucose level were higher by 2.7 times in DM+N group and suggested about worsening of glycemia by accompanied neurodegeneration. Activity of GLUT4 in brain hyppocampus were decreased by 15% in DM and 19% in DM+N group in compare with control rats suggesting about poor insulin signaling in brain hyppocampus and were more worsen in combination of neurodegeneration.

Conclusion: Insulin signaling transduction were affected in experimental DM and accompanied with decreasing of GLUT4 activity in brain hyppocampus. Neurodegeneration more worsen insulin signaling transduction and more affects GLUT4 activity in rats brain hyppocampus.