Endocrine Abstracts

JOINT2982

Obeject: To explore the effect and potential mechanisms of balanced diet combined with high-intensity interval training (HIIT) in reducing fat mass and increasing muscle mass in overweight and obese children.

Methods: Totally 44 obese and 10 overweight children aged from 8 to 18 years old were recruited to participate in a 3-week weight loss summer camp, all the subjects received the same dietary regimen and HIIT. We compared the changes in physical parameters, body composition (DXA), biochemical parameters (including OGTT test) and liver CAP (Fibroscan) in all the subjects, additionally, serum metabolomic and lipidomic profiles, gut microbiomes were further investigated in 8 of them at the beginning and the end of the summer camp.

Results: The average weight of the participants decreased by 3.29kg, BMI by 1.16kg/m2, waist circumference by 3.1cm, liver CAP by 8.44, but the difference was not statistically significant. The total fat mass decreased from (27278.32+8133.78) to (24275.68+6871.31), while total lean mass increased from (32652.02+8495.73) to (35210.37+8465.29) (p<0.05). ALT, uric acid, GGT, TC, LDL, Apolipoprotein A1/B, HOMA-IR were significantly improved after the summer camp (p<0.05). Serum metabolisms revealed 27 differential metabolites, serum lipidomic revealed 21 differential lipids, gut microbiomes revealed 60 differential species (p<0.05), enrichment analysis revealed that the differential metabolites, lipids and gut microbiotas were involved in sphingolipid metabolism, glycerophospholipid metabolism, glycosylphosphatidylinositol-anchor biosynthesis, cysteine and methionine metabolism and primary bile acid biosynthesis, which were involved in cell autophagy and associated with insulin resistance.

Conclusion: Three weeks of balanced diet and HIIT can significantly reduce the total fat mass, increase total lean mass, and improve the glucose and lipid metabolism indicators among obesity and overweight children. Mult-omics sequencing is helpful to find early metabolite changes and explore the potential mechanisms during weight loss in children.