IES2025 Research, Audit and Quality Improvement Projects Physical Posters (55 abstracts)
Review of a Serum Thyroglobulin Laboratory Service using a High-Sensitivity Brahms Kryptor® Immunoassay in an Irish Academic Teaching Hospital
Ciara De Buitléir , Robert O’Byrne , Aoife McConnon , Micheál Mac Aogáin , Sanja Cumpf , Philip Holmes , Margaret McCormack , Marie-Louise Healy & Vivion Crowley
St. James’s Hospital, Dublin, Ireland
Expert consensus guidelines recommend the use of high-sensitivity assays for thyroglobulin measurement and the concomitant analysis of thyroglobulin antibody (TgAb) levels to gauge potential Tg assay interference. We provide a review of the service operated by St James’s Hospital, Dublin using the Brahm’s Kryptor assay (limit of quantitation <0.15µg/l), which also measures Tg recovery (Tg-mRec). Data for patients >16 years of age covering 01.01.22 to 22.07.2025 was collated and analysed using Excel. A total of 8,669 samples had both Tg and TgAb assayed, which represented 3,102 patients (75.2% female), with an average repeat rate of 2.6/patient (range 1-37). Overall, 43.3% of samples had Tg < 0.15µg/l, with a Tg range (<0.15 –160,439), while 31.1% of samples were TgAb-positive (range 27–39,444U/mL). Of note 27% of all samples had Tg <0.15 and were TgAb positive, which could potentially contribute to falsely low Tg results. To assess the impact of TgAb status on Tg-mRec, a subset of data covering a 6-month period was analysed (n = 1,237 samples, n = 943 patients). In this subset, 2.5% (n = 29) of all samples failed Tg-mRec, while in 29% of TgAb-positive samples (n = 362) only 7.5% (n = 27) failed. Notably, 0.2% (n = 2) of TgAb-negative samples (n = 870) were assigned as failing Tg-mRec. Moreover, 0.4% of all samples had an elevated Tg-mRec (>120%), suggestive of heterophile antibody interference. This service review indicates that serum Tg levels and TgAb-positive rates are consonant with other reported series. The Tg-mRec data suggest that marked assay interference may only manifest in a very small subset of samples. doi.org/10.1093/ejendo/lvad109
Volume 115
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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