Searchable abstracts of presentations at key conferences in endocrinology

ea0016p693 | Thyroid | ECE2008

Effects of 8-Cl-cAMP on growth and apoptotic process in poorly differentiated thyroid cancer cell lines

Lucchi Simona , de Filippis Tiziana , Calebiro Davide , Porazzi Patrizia , Spada Anna , Persani Luca

Tools that are highly effective in the treatment of differentiated thyroid cancer (DTC) loose their therapeutic potentials in poorly differentiated tumors. The synthetic analog 8-Cl-cAMP has been known to have an antiproliferative effect in a variety of cancer cells and is tested as antineoplastic agent in clinical trials. The signaling mechanisms that govern the 8-Cl-cAMP-induced growth inhibition are still uncertain and data in thyroid neoplasia are lacking. Therefore, we te...

ea0011oc61 | ThyroidOC57 British Thyroid Association Award | ECE2006

Phosphodiesterase-resistant PKA I agonists inhibit thyroid cancer cell growth

Lucchi S , Calebiro D , de Filippis T , Porazzi P , Beck-Peccoz P , Persani L

In thyroid cells TSH/cAMP stimulation is associated with proliferation and differentiation. The principal effector of cAMP is the protein kinase A (PKA) and events downstream PKA activation, signaling specificity and expression of PKA isoforms are largely unexplored. PKA is composed of two regulatory and two catalytic subunits. Two major isoforms of PKA (I and II) are determined by their specific regulatory subunits (RIα, RIβ, RIIα and RIIβ). A recent gene ...

ea0011p887 | Thyroid | ECE2006

PKA II mediates cAMP-dependent phosphorylation of CREB and activation of gene transcription in differentiated rat thyroid cells

Calebiro D , de Filippis T , Lucchi S , Porrazzi P , Beck-Peccoz P , Persani L

Thyroid stimulating hormone (TSH) is the key modulator of thyroid cell function. Binding of TSH to its receptor (TSHR) results in an increase of cAMP intracellular concentration, which activates protein kinase A (PKA) to phosphorylate CREB and other substrates. Eventually, this leads to gene transcription modification associated with both proliferation and differentiation. Two major types of PKA (PKA I and II) exist in mammal cells, but their specific role in TSH signaling is ...