Searchable abstracts of presentations at key conferences in endocrinology

ea0022p350 | Diabetes | ECE2010

The GG genotype of the G-11391A ADIPOQ polymorphism might be a risk factor for development of type 2 diabetes mellitus in women

Polosak Jacek , Roszkowska-Gancarz Malgorzata , Kurylowicz Alina , Turowska Olga , Franek Edward , Puzianowska-Kuznicka Monika

Background: Adiponectin is a protein hormone secreted by adipocytes. Its expression is higher in lean than in obese individuals and in women than in men. It inhibits expression of gluconeogenic enzymes and the rate of glucose production. It also stimulates glucose utilization and fatty acids oxidation. Adiponectin is encoded by a highly polymorphic ADIPOQ gene. The −11377C>G, −11391G>A, and −11426A>G ADIPOQ polymorphisms modulate cir...

ea0022p653 | Neuroendocrinology and Pituitary (<emphasis role="italic">Generously supported by Novartis</emphasis>) | ECE2010

Leptin receptor gene expression and its promoter methylation do not change with age in peripheral blood mononuclear cells

Roszkowska-Gancarz Malgorzata , Owczarz Magdalena , Polosak Jacek , Kurylowicz Alina , Puzianowska-Kuznicka Monika

Introduction: Leptin is a protein hormone secreted mainly by adipose tissue. It exerts its function via ubiquitously expressed receptors (LEPR). A number of LEPR isoforms are known. Metabolism of lipids and carbohydrates, that, in part, depends on leptin, might deteriorate with age. Aging is associated with a decrease of the global methylation and with hypermethylation of some promoter-located CpG islands. Such hypermethylation results in transcription inhibition.<p class=...

ea0035p783 | Obesity | ECE2014

Decreased expression of genes encoding thermogenesis-related proteins in adipose tissues of obese patients is not associated with their methylation status

Kurylowicz Alina , Jonas Marta , Lisik Wojciech , Jonas Maurycy , Wicik Zofia Agnieszka , Wierzbicki Zbigniew , Chmura Andrzej , Puzianowska-Kuznicka Monika

Introduction: Thermogenesis constitutes an important part of energy expenditure and its disturbances may contribute to the development of obesity. The aim of this study was to investigate whether the expression of thermogenesis-related genes differs in adipose tissues of obese and normal-weight individuals and if methylation of CpG islands located in the regulatory regions of these genes is involved in this phenomenon.Materials and methods: The expressio...

ea0035p784 | Obesity | ECE2014

Different relationship between serum 25OH-vitamin D and expression of vitamin D 25-hydroxylase gene CYP2R1 in subcutaneous adipose tissue of obese and slim subjects.

Jonas Marta , Kurylowicz Alina , Bartoszewicz Zbigniew , Lisik Wojciech , Jonas Maurycy , Wierzbicki Zbigniew , Chmura Andrzej , Puzianowska-Kuznicka Monika

Introduction: In Central Europe, vitamin D deficiency is common, and a very pronounced deficiency of this vitamin is observed in obese individuals. We hypothesized that the decreased serum concentration of vitamin D might be in part due to the changed metabolism of this vitamin in adipose tissues.Methods: We assessed the expression of vitamin D 25-hydroxylase genes CYP2J2 and CYP2R1 in adipose tissues of obese and slim patients using RT...

ea0035p1060 | Thyroid (non-cancer) | ECE2014

THRB expression is affected by miR-26a and miR-496, but age-associated decrease of the expression of this gene in human peripheral blood mononuclear cells is not dependent on these miRNAs

Pawlik-Pachucka Eliza , Budzinska Monika , Domaszewska-Szostek Anna , Owczarz Magdalena , Gewartowska Magdalena , Kurylowicz Alina , Puzianowska-Kuznicka Monika

Introduction: Decreased function of the thyroid axis during aging is associated with a better functional performance and longer survival. This phenomenon can be a result not only of a lower production of thyroid hormones including bioavailable triiodothyronine, but also of age-associated alterations regarding triiodothyronine receptors.Methods: Functional studies regarding interaction between the 3′UTR of TRβ1 mRNA and in silico-ident...

ea0016p165 | Comparative endocrinology | ECE2008

Chemokine CXCL10 gene polymorphisms in Addison's disease

Sadet Dilek , Penna-Martinez Marissa , Kurylowicz Alina , Paunkovic Jane , Kahles Heinrich , Bruck Patrick , Badenhoop Klaus , Ramos-Lopez Elizabeth

Background and aims: CXC chemokine ligand 10 (CXCL10) also known as chemokine interferon γ inducible protein (IP-10) is a CXCR3 chemokine belongs to a group of structurally related molecules, that induce the chemotaxis of diverse leukocyte subtypes including activated T-helper 1 lymphocytes, natural killer cells and monocytes. In patients with Hashimoto’s thyroiditis, type 1 diabetes mellitus and Graves’ disease high levels of CXCL10 ligand in serum have been fo...