Searchable abstracts of presentations at key conferences in endocrinology

ea0016p27 | Adrenal | ECE2008

Modulation of adrenocortical aldosterone and cortisol synthesis by in vitro oxidized low density lipoprotein

Kopprasch Steffi , Ansurudeen Ishrath , Graessler Juergen , Bornstein Stefan R , Pietzsch Jens

Objectives: Oxidative stress is of critical importance in the pathogenesis of endocrinopathies. Since cholesterol serves as a major source of steroid hormone synthesis we investigated the effect of hypochlorite-modified low density lipoprotein (LDL) on aldosterone and cortisol release from human adrenocortical NCI-H295R cells.Methods: Native LDL obtained from healthy volunteers was oxidized to varying degrees by sodium hypochlorite. The resulting modifie...

ea0022p64 | Adrenal | ECE2010

Endothelial cell-derived products regulate β-catenin-dependent transcription in human adrenocortical cells

Schwafertz Carolin , Schinner Sven , Kuhn Markus , Ansurudeen Ishrath , Oetjen Elke , Schott Matthias , Scherbaum Werner A , Willenberg Holger S

Objective: Recently, we have shown that endothelial cell-conditioned medium (ECCM) stimulates aldosterone secretion and the expression of proteins, essential for steroidogenesis and adrenal development. Also, we have found that fat cells regulate adrenal steroidogenesis through the wnt-signaling pathway. In addition, β-catenin was implicated in adrenal tumorigenesis, including aldosterone-producing adenomas.Methods: We studied the influence of endot...

ea0016p59 | Adrenal | ECE2008

Evidence for the involvement of endothelial cell products in adrenal CITED2 expression

Haase Matthias , Ansurudeen Ishrath , Schinner Sven , Paramonova Iryna , Schott Matthias , Papewalis Claudia , Krug Alexander , Scherbaum Werner A , Willenberg Holger S

The mechanisms that lead to tumor formation of and aldosterone secretion by zona glomerulosa cells in primary aldosteronism are not known. Recently, endothelial cell-derived factors have been characterized that control the release of aldosterone by adrenocortical cells. In addition, we started to characterize the regulation of CITED2, a CBP/p300 interacting transactivator with ED-rich tail 2. Earlier, it was shown in mice that absence of CITED2 leads to adrenal agenesis. Since...