Searchable abstracts of presentations at key conferences in endocrinology

ea0016p540 | Obesity | ECE2008

Retinol-binding protein 4 is independently associated with insulin resistance in PCOS women

Mohlig Matthias , Weickert Martin O , Ghadamgahi Elham , Arafat Ayman M , Spranger Joachim , Pfeiffer Andreas FH , Schofl Christof

Objective: Adiposity, insulin resistance, and hyperandrogenism are features of the polycystic ovary syndrome (PCOS). Retinol-binding protein 4 (RBP4) secreted from adipose and liver tissue has been linked to insulin resistance. We here address the impact of RBP4 on insulin resistance in PCOS and its usability to identify women with metabolic syndrome or impaired glucose metabolism (IGT or diabetes).Design: RBP4 was determined in plasma of 115 PCOS...

ea0014p19 | (1) | ECE2007

Insulin decreases IGF-I bioactivity in patients with impaired glucose tolerance and in healthy subjects

Arafat Ayman M , Frystyk Jan , Weickert Martin O , Spranger Joachim , Schöfl Christof , Möhlig Mathias

Objectives: Insulin resistance (IR) is a very common metabolic abnormality in obesity, which is often associated with reduced growth hormone (GH) secretion. GH deficiency is associated with increased in intra-abdominal fat and several parameters of the metabolic syndrome. IGF-I improves IR but the IGF-binding proteins are supposed to regulate its bioactivity although only little information exists. We postulated that the elevated insulin levels due to IR do not only suppress G...

ea0037gp.17.03 | Pituitary–Neuroendocrinology and central salt regulation | ECE2015

Insulin-induced hypoglycaemia decreases IGF1 bioactivity in humans: a missing link to increased mortality in diabetic patients?

Sarem Zeinab , Assefa Biruhalem , Weickert Martin O , Adamidou Aikatarini , Frystyk Jan , Mohlig Matthias , Lieske Stefanie , Birkenfeld Andreas L , Pfeiffer A.F.H , Spranger Joachim , Arafat Ayman M

Background: The mechanisms underlying the association between severe hypoglycaemia and increased cardiovascular mortality among patients with diabetes mellitus are not fully understood. Our aim was to evaluate changes in GH/IGF1 axis during insulin-induced hypoglycaemia, as a possible link.Methods: Twenty-five healthy subjects, 12 obese participants (OP; 6/ 6; 34.4±1.7 kg/m2), and 13 healthy lean participants (LP; 6/ 7; 21.7±0.6 kg/m...

ea0016p439 | Neuroendocrinology | ECE2008

A comparison between three automated chemiluminescence assays for growth hormone: on the use of recombinant hGH as a primary calibrant

Arafat Ayman M , Mohlig Matthias , Weickert Martin O , Perschel Frank H , Purschwitz Johannes , Spranger Joachim , Strasburger Christian J , Schofl Christof , Pfeiffer Andreas FH

Objectives: GH measurements during OGTT have profound effects on therapy and follow-up management of acromegaly. To minimize the discordance between immunoassays, it is recommended to calibrate them using 22 kDa-GH-preparation instead of the use of pituitary-derived calibrants. The aim of our study was to evaluate the between-method discrepancies in GH determinations by assays using different calibrants considering further confounders like age, gender, and BMI.<p class="ab...

ea0016p541 | Obesity | ECE2008

Glucagon suppression of total but not of acylated ghrelin is preserved in obesity: the impact on appetite control

Arafat Ayman M , Adamidou Aikaterini , Otto Barbel , Weickert Martin O , Perschel Frank H , Spranger Joachim , Schofl Christof , Mohlig Matthias , Pfeiffer Andreas F H

Objective: The mechanisms underlying the well known glucagon-induced satiety effect are unclear. As we showed recently, the glucagon-induced reduction in total ghrelin, that might be responsible for this effect, is exerted at hypothalamus–pituitary level. The aim of the present study was to further evaluate glucagon’s suppressive effect on both total and acylated-ghrelin in obesity with respect to its role in appetite control.Methods: Prospecti...

ea0014oc8.1 | Neuroendocrinology clinical | ECE2007

Growth hormone response during OGTT: the impact of assay method, gender and BMI on the estimation of reference values in patients with acromegaly and in healthy controls

Arafat Ayman M , Möhlig Mathias , Weickert Martin O , Perschel Frank H , Purschwitz Johannes , Spranger Joachim , Strasburger Christian J , Schöfl Christof , Pfeiffer Andreas FH

Objectives: Besides the measurement of IGF-1, GH suppression during OGTT to assess the biochemical status in acromegaly is recommended. However, as a consequence of the development of highly sensitive and specific GH assays a critical re-evaluation of the criteria for the diagnosis and follow-up management of acromegaly is mandatory. The aim of our study was to evaluate the between-method discrepancies in GH determinations by different immunoassays considering further confound...