Searchable abstracts of presentations at key conferences in endocrinology

ea0040oc5 | (1) | ESEBEC2016

The anti-proliferative effect of metformin in a model of adrenocortical carcinoma

Armignacco Roberta , Poli Giada , Cantini Giulia , Canu Letizia , Mannelli Massimo , Luconi Michaela

Adrenocortical carcinoma (ACC) is a rare, heterogeneous malignancy with aggressive behavior and poor prognosis, particularly when metastatic at diagnosis. To date, radical surgery, possibly associated to mitotane adjuvant therapy, is considered the best option for ACC treatment. However, the mean 5-year survival rate diminishes dramatically in metastatic ACC and chemo-resistance often develops. Thus, more specific and effective drugs for ACC treatment are urgently required. Th...

ea0037ep68 | Adrenal cortex | ECE2015

The antidiabetic drug metformin affects H295R cells proliferation

Armignacco Roberta , Poli Giada , Cantini Giulia , Canu Letizia , Luconi Michaela , Mannelli Massimo

Adrenocortical carcinoma (ACC) is a rare, heterogeneous malignancy with a poor prognosis, particularly when metastatic at diagnosis. To date, radical surgery, possibly associated to mitotane adjuvant therapy, is the only available treatment. However, the mean 5-year survival rate drops under 10% in metastatic ACC and chemo-resistance often develops. Thus, more specific and effective drugs for ACC treatment are urgently required. The antidiabetic drug metformin, used in type 2 ...

ea0070oc1.3 | Adrenal and Cardiovascular Endocrinology | ECE2020

Tumor microenvironment adipose stem cells modulate adrenocortical carcinoma progression

Cantini Giulia , Armignacco Roberta , de Assis Goncalves Diego , Guasti Daniele , Nesi Gabriella , Romagnoli Paolo , Mannelli Massimo , Maggi Mario , Luconi Michaela

The metabolic interplay occurring between the tumor microenvironment and cancer cells may represent a potential target for novel anti-cancer approaches. Among stromal components, adipocytes and adipose precursors have been shown to actively participate in tumor progression in several solid malignancies. In adrenocortical carcinoma (ACC), a rare endocrine neoplasia with a poor prognosis, cancer cells often infiltrate the fat mass surrounding the adrenal organ, enabling a possib...

ea0081p543 | Adrenal and Cardiovascular Endocrinology | ECE2022

Whole blood transcriptomic profile of Cushing’s syndrome

Armignacco Roberta , Daniel De Murat , Jouinot Anne , Bouys Lucas , Perlemoine Karine , Letourneur Franck , Adoux Lucie , Zennaro Maria-Christina , Bertherat Jerome , Assie Guillaume

Background: Cushing’s syndrome, caused by an excess of circulating glucocorticoids, is associated with high morbidity and presents high inter-individual variability. The earlier the diagnosis, the better the treatment effectiveness and the prognosis. Hormone assays, routinely used, contribute to identify Cushing’s syndrome. However, no biomarker is currently available to directly quantify the biological action of glucocorticoids. Blood samples represent an easily obt...

ea0063oc13.5 | Anterior and Posterior pituitary 2 | ECE2019

Pan-genomic classification of pituitary adenomas

Neou Mario , Villa Chiara , Armignacco Roberta , Jouinot Anne , Septier Amandine , Raffin-Sanson Marie-Laure , Perlemoine Karine , Bernier Michele , Baussart Bertrand , Bertherat Jerome , Gaillard Stephan , Assie Guillaume

Pituitary adenomas, now called pituitary neuroendocrine tumors (PitNETs), vary in histological type, secretion, invasion and growth speed. A World Health Organization (WHO) histo-prognostic classification was released in 2017. Pituitary tumorigenesis is largely unexplained. Rare germline mutations (MENIN, AIP), and common somatic mutations in somatotroph (GNAS) and corticotroph (USP8) are reported. Recently, genomic analyses have been report...

ea0063gp243 | Anterior and Posterior Pituitary | ECE2019

Histological classification of pituitary neuroendocrine tumors: genomic insights on cell lineage

Villa Chiara , Neou Mario , Armignacco Roberta , Jouinot Anne , Septier Amandine , Perlemoine Karine , Bernier Michele , Laure Raffin-Sanson Marie , Baussart Bertrand , Bertherat Jerome , Gaillard Stephan , Assie Guillaume

The 2017 World Health Organization (WHO) classification of pituitary adenomas is based on cell lineage and transcription factors (TFs). Pituitary progenitors expressing Pit-1 are driven towards the somato-lacto-thyrotroph differentiation, T-Pit towards corticotroph, and SF-1 towards gonadotroph. We recently generated a multi-genomic classification of pituitary neuroendocrine tumors (PitNETs) (abstract submitted to ECE2019 by Neou M.). Transcriptome classification identified si...

ea0063p452 | Adrenal and Neuroendocrine Tumours 2 | ECE2019

Identification of a molecular signature of hypercortisolism by whole blood methylome analysis

Armignacco Roberta , Septier Amandine , Gaspar Cassandra , Jouinot Anne , Neou Mario , Perlemoine Karine , Zennaro Maria Christina , Reincke Martin , Bertherat Jerome , Beuschlein Felix , Assie Guillaume

The prolonged exposure to an excess of circulating cortisol (Cushing’s syndrome) causes various complications. An accurate and early diagnosis is critical for effective surgical management and optimal prognosis. However, the current diagnostic approach based on hormonal assays can be complex and requires multiple tests. The identification of novel, specific and easily measurable biomarkers of hypercortisolism may help to improve the diagnosis and to evaluate the complicat...

ea0049gp8 | Adrenal 1 | ECE2017

Clinical and translational relevance of circulating miR483 in adrenocortical cancer

Canu Letizia , Salvianti Francesca , Poli Giada , Armignacco Roberta , Cantini Giulia , Di Franco Alessandra , Gelmini Stefania , Ercolino Tonino , Terzolo Massimo , Nesi Gabriella , Pinzani Pamela , Mannelli Massimo , Luconi Michaela

Adrenocortical cancer (ACC) is a rare aggressive malignancy, with poor prognosis when metastaic at diagnosis. Recent ACC pan-genomics analysis contributed to redefine the risk groups on molecular bases, including tumor micro RNA (miR), which can be detectable not only in the primary lesion but also in the bloodstream.We develop a quantitative real-time assay for the measurement of absolute levels in plasma samples of miR483 and its mature miR483-5p form....

ea0070oc4.3 | Pituitary and Neuroendocrinology | ECE2020

Clinical implications of Pan-genomic classification of pituitary neuroendocrine tumours

Villa Chiara , Neou Mario , Armignacco Roberta , Anne Jouinot Anne , Raffin-Sanson Marie-Laure , Septier Amandine , Perlemoine Karine , Bernier Michèle , Baussart Bertrand , Bertherat Jerome , Gaillard Stéphan , Assié Guillaume

Recently, we provided the first integrated genomic classification of pituitary neuroendocrine tumors (PitNETs), on a series of 134 tumors. This series covered all histological, secretion, invasion and growth speed types. This molecular classification supports the importance of pituitary lineage as proposed by the World Health Organization 2017 classification, but also individualizes new entities. Indeed, corticotroph tumors are split into three distinct molecular groups. In ad...

ea0070yi12 | Young Investigators | ECE2020

Identification of transcriptome profiles in paraffin samples using 3’ RNA-sequencing for the prognostic assessment of adrenocortical carcinoma

Jouinot Anne , Sibony Mathilde , Jeanpierre Lindsay , Septier Amandine , De Murat Daniel , Armignacco Roberta , Perlemoine Karine , Izac Brigitte , Letourneur Franck , Ragazzon Bruno , Leroy Karen , Gaujoux Sebastien , Dousset Bertrand , Groussin Lionel , Libe Rossella , Bertherat Jerome , Assié Guillaume

Background: Adrenocortical cancer (ACC) is an aggressive tumor with heterogeneous prognosis. Previous genomic studies have demonstrated the importance of molecular classification for the prognostic assessment. Among molecular markers, transcriptome profiles “C1A” (steroid and proliferative signature) and “C1B” (immune signature) show the strongest association with outcome. However these markers are determined so far only from frozen tissue samples, sinc...