Searchable abstracts of presentations at key conferences in endocrinology

ea0044ep37 | (1) | SFEBES2016

Spurious hypoglycaemia caused by an IgM paraprotein

Armston Annie , Zarif Nadia , Meeking Daryl

Mrs IP is a 79 year old lady with a past medical history of spleenic marginal zone lymphoma, diagnosed in 2007 and treated with chemotherapy. She has suffered two relapses but despite her diagnosis had remained generally quite fit and healthy until the recent issues. An IgM paraprotein was identified in the patient’s serum an December 2014.In April 2015 Mrs IP presented to her GP complaining of cramping symptoms overnight, especially marked in her h...

ea0028p382 | Thyroid | SFEBES2012

Removal of heterophilic antibody interference in thyroid function tests with heterophilic blocking tubes

Armston Annie , Hyde Sarah , Day Linda

Heterophilic antibodies are human immunoglobulins with antigenic determinants directed against non-human species. These antibodies can interfere with the measurement of analytes in routine clinical laboratories, in particular in 2-site immunoassays. Skybio heterophilic blocking tubes-50 (HBT-50) are one way of overcoming this interference but, it has been reported (Sturgeon and Viljoen, Annals Clin Biochem, 2011, 48, 418), some samples may require two treatments to completely ...

ea0034p362 | Steroids | SFEBES2014

An audit on the outcomes of bloodspot 17-OHP results on the management of patients with CAH

Mozley Erin , Salter Simon , Davies Justin , Armston Annie

Introduction: Measurement of bloodspot 17-OHP is the current method of assessing hydrocortisone replacement in patients with congenital adrenal hyperplasia (CAH). Bloodspot 17-OHP has been measured in Southampton by LC–MS/MS since August 2011. Clinical observations of signs and symptoms of under- and over-treatment are equally as important.The aim of this audit was to assess whether the 17-OHP profile results are being appropriately used for adjustm...

ea0038p24 | Clinical biochemistry | SFEBES2015

Serum cortisol: what is your laboratory measuring?

Hawley James , Owen Laura , Monaghan Phillip , Armston Annie , Chadwick Carrie , Keevil Brian

Background: Accurate measurement of serum cortisol is essential in the investigation of the HPA axis. It has been documented that routine immunoassays are liable to both under- and over-recovery leading to inaccurate results and subsequent inappropriate investigations. This study seeks to provide an up-to-date assessment of the accuracy of the major immunoassay platforms and compares results to a liquid chromatography–tandem mass spectrometry (LC–MS/MS) candidate ref...

ea0036oc4.7 | Oral Communications 4 | BSPED2014

Pitfalls in the diagnosis of neonatal adrenal insufficiency

Irvine Vanessa , Davis Nikki , Walker Jo , Wickramasuriya Nalin , Cook Paul , Armston Annie , Davies Justin

Introduction: Adrenal insufficiency is rare in the neonatal period and if unrecognised may cause life-threatening circulatory collapse. The initial investigations taken at the time of presentation, and prior to the institution of hydrocortisone, are a key step in the diagnostic pathway, and aid the clinician to distinguish adrenal insufficiency from mineralocorticoid resistance or renal tubulopathy. A cortisol measurement at the time of illness is useful to evaluate the adrena...

ea0038p22 | Clinical biochemistry | SFEBES2015

Effect of cortisol assay bias on the overnight dexamethasone suppression test: implications for the investigation of Cushing’s syndrome

Brixey-McCann Rachel , Tennant Sarah , Geen John , Armston Annie , Barth Julian H , Keevil Brian , Rees Aled , Evans Carol

Background: NEQAS data demonstrate a divergence in bias of cortisol immunoassays over the last 10 years. Despite this, a serum cortisol of 50 nmol/l has been universally applied as the cut-off for the overnight dexamethasone suppression test (ONDST), the commonest screening test for Cushing’s syndrome.Aims: To assess the effect of assay bias on interpretation of the ONDST and determine the necessity for a method-specific cut-off.<p class="abstex...