Searchable abstracts of presentations at key conferences in endocrinology

ea0019p114 | Cytokines and growth factors | SFEBES2009

Expression and purification of a potential long-acting leptin agonist

Carpenter B , Pradhananga S , Wilkinson I , Justice S , Artymiuk P , Ross R

Background: Leptin is an important regulator of the immune response. Therapeutics that modulate leptin signalling are considered to be a potential targets in a number of immune related conditions. The role of leptin antagonists to suppress the immune system, slowing the progression of autoimmune diseases is a widely discussed idea. However, leptin agonists may also be useful to up-regulate the immune response during malnutrition. The aim of this project was to develop long-las...

ea0005p71 | Cytokines and Growth Factors | BES2003

A growth hormone receptor (GHR)- glycophosphatidylinositol (GPI) chimera acts as a receptor antagonist

Gavalas N , Pradhananga S , Beghadi C , Maamra M , Zarkesh-Esfahani H , Sayers J , Artymiuk P , Reymond C , Ross R

We have previously demonstrated that a truncated GHR lacking the cytoplasmic domain of the receptor acts as a dominant negative regulator of receptor signalling. Glycosylphosphatidylinositol (GPI) anchors are a common mechanism by which cells direct and anchor proteins in the cell membrane. We have generated a GHR extracellular domain-GPI fusion protein and tested its biological activity as a receptor antagonist. The extracellular domain of GHR (growth hormone binding protein-...

ea0007p26 | Cytokines and growth factors | BES2004

GHR-GPI insertion into target CHO cell membranes

Gavalas N , Zarkesh-Esfahani S , Wilkinson I , Pradhananga S , Beghadi C , Baumann G , Wu Z , Artymiuk P , Sayers J , Ross R

Introduction: We have generated a truncated Growth Hormone Receptor (GHR) fused to the cell membrane by a glycosyl phosphatidylinositol anchor (GHR-GPI). The protein consists of the complete extracellular domain of the GHR, but lacks the intracellular domain, and we predict it would act as a GH antagonist. Aim: To test if partially-purified soluble GHR-GPI could re-insert into CHO cell membranes. Material and methods: Membrane preparations were made from CHO cells stably expre...