Searchable abstracts of presentations at key conferences in endocrinology

ea0059p168 | Obesity & metabolism | SFEBES2018

The impact of freeze dried broccoli extract to mitigate inflammation in human adipocytes through the mevalonate pathway

Murphy Alice , Azharian Sahar , Tripathi Gyanendra , Barker Guy , Chappell Michael , McTernan Philip G

Background: Delivery of nutrient excess in obesity can disrupt protein folding in the endoplasmic reticulum (ER) within adipose tissue; this activates the unfolded protein response (UPR) and contributes to type 2 diabetes mellitus (T2DM) risk. Thus, the aims of this study were to utilise freeze-dried broccoli extract (BE) as a nutrient to mitigate such cellular damage in human adipocytes, understand the relevance of associated pathways, and create a mathematical model of the U...

ea0044p110 | Diabetes and Cardiovascular | SFEBES2016

Volatile organic compounds: A potential biomarker for prediction of hypertension

Azharian Sahar , Neal Matthew , Kyrou Iannos , Piya Milan , Kumar Sudesh , Covington James , McTernan Philip , Vrbikova J , Hainer V , Sramkova P , Fried M

Background: Human obesity can significantly contribute to hypertension risk, often referred to as the ‘silent killer’, although targeting whom with weight gain becomes clinically hypertensive is unclear. Whilst therapeutic intervention such as bariatric surgery can mitigate both obesity and hypertension, it is not the most suitable solution for all patients. Therefore an early biomarker detection system to assess hypertension in subjects with weight gain could reduce...

ea0044p191 | Obesity and Metabolism | SFEBES2016

Freeze dried broccoli extract relieves ER stress and mitochondrial inefficiency in differentiated human pre-adipocyte cells

Murphy Alice , de la Escalera Clapp Lucia Martinez , Jackisch Laura , Azharian Sahar , Aladel Alanoud , Barker Guy , Tripathi Gyanendra , Chappell Michael , McTernan Philip

Background: In obesity, excess nutrients can disrupt protein folding in the endoplasmic reticulum (ER) which activates the unfolded protein response (UPR) and alters mitochondrial function. These changes can induce inflammation, oxidative stress and insulin resistance. The aim of the study was to investigate whether broccoli extract can protect against cellular damage in human adipocytes, which with mathematical modelling may help predict pathway response.<p class="abstext...

ea0038fp7 | (1) | SFEBES2015

Regulation of adipogenesis by wnt10b signalling in cultured human adipocytes taken from an obese patient with a WNT10B-C256Y mutation

Azharian Sahar , Voyias Philip D , Murphy Alice , de la Escalera Clapp Lucia Martinez , Adaikalakoteswari Antonysunil , Saravanan Ponnusamy , Piya Milan K , McTernan Philip G , Tripathi Gyanendra

Background: Adipogenesis is a key process leading to adipose tissue (AT) expansion. However, managing this event is critical to limit excessive fat accumulation and disease risk. Adipogenesis appears controlled in part, through Wnt10b signalling which reduces adipogenesis via inhibiting CCAAT/enhancer-binding protein alpha (CEBPα) although the molecular mechanism remains unclear.Aims: Abdominal subcutaneous AT (AbdSc AT) was collected from a, non-di...