Searchable abstracts of presentations at key conferences in endocrinology

ea0037ep1220 | Clinical Cases–Pituitary/Adrenal | ECE2015

Occurrence of liver failure in post-surgery hypopituitaric patients

Lo Preiato Valentina , Ribichini Danilo , Baccini Margherita , Pasquali Renato , Pagotto Uberto

Multiple pituitary hormone deficit and hypothalamic obesity are common complications after brain surgery for childhood tumors. Despite adequate replacement hormone therapy, obesity develops equally, but liver failure is not usually described. In this study, four subjects who had undergone surgery for brain tumors involving the peri-hypothalamic area when they were nursing or as youths (9 months, 6 years, 9 years and 20 years). They were referred to our Unit when they were 19, ...

ea0035p757 | Obesity | ECE2014

Circulating endocannabinoids are associated with cardiometabolic impairment independently of adiposity in normal weight subjects

Fanelli Flaminia , Belluomo Ilaria , Cuomo Gaia , Mezzullo Marco , Baccini Margherita , Casadio Elena , Pasquali Renato , Pagotto Uberto

Obesity is characterized by hyperactivated endocannabinoid System (ECS) in brain and periphery and by increased levels of circulating endocannabinoids (cECs), anandamide (AEA) and 2-arachidonoylglycerol (2AG). It is still debated whether in absence of obesity cECs are only modulated by adiposity or if they are associated with cardiometabolic parameters.We recruited 155 healthy normal weight (NW, BMI: 18.5–24.9 kg/m2) drug-free volunteers ...

ea0049ep669 | Obesity | ECE2017

Menopause is a major determinant of endocannabinoid 2-arachidonoylglycerol plasma level and of its relevance as biomarker of dyslipidemia and insulin resistance in lean women

Fanelli Flaminia , Mezzullo Marco , Baccini Margherita , Casadio Elena , Gasparini Daniela Ibarra , Vicennati Valentina , Pasquali Renato , Pagotto Uberto

Context: The endocannabinoid system (ECS) hypertonicity contributes to obesity development and maintenance. Excess circulating levels of ECS mediator 2-arachidonoylglycerol (2AG) were reported in obese humans, however, unstandardized experimental settings (i.e. analytical conditions, sample size, concurrent diseases/medications, statistics, gender, body mass index (BMI) and age), clouded its role in dysmetabolism and its usefulness as biomarker. The study aimed at describing 2...

ea0049ep671 | Obesity | ECE2017

Endocannabinoid 2-arachidonoylglycerol plasma level is a BMI-independent biomarker of cardiometabolic risk in adult males with higher relevance in lean and aged conditions

Fanelli Flaminia , Mezzullo Marco , Baccini Margherita , Casadio Elena , Gasparini Daniela Ibarra , Vicennati Valentina , Pasquali Renato , Pagotto Uberto

Context: Ageing and obesity are similarly characterized by metabolic impairment in terms of visceral fat, dyslipidaemia, insulin resistance and hypertension. Obesity is featured by endocannabinoid system (ECS) hyperactivation. Increased levels of endocannabinoid 2-arachidonoylglycerol (2AG) found in obesity were variously associated to dysmetabolic features, however, it is not clear whether such associations exist in normal weight conditions. Moreover, ageing contribute in mod...

ea0041oc10.5 | Reproduction & Endocrine Disruption | ECE2016

Effect of clomiphene citrate and metformin on testosterone levels in hypogonadal obese men with impaired glucose tolerance (IGT) or type 2 diabetes (DM2)

Pelusi Carla , Giagulli Vito , Baccini Margherita , Fanelli Flaminia , Mezzullo Marco , Fazzini Alessia , Casadio Elena , Prontera Olga , Pagotto Uberto , Pasquali Renato

Low testosterone (T) levels are often found in obese men with metabolic abnormalities, however the mechanism underling this condition is unclear. This study aimed to evaluate the effectiveness of clomiphene citrate (CC) in increasing T levels and improving metabolic control in hypogonadal obese men with IGT or DM2. A randomized cross-over double blind controlled study was conducted in twenty obese hypogonadal caucasian men, mean age 47 ys, classified in IGT (n=11) or ...

ea0041ep658 | Female Reproduction | ECE2016

Sex hormone and steroid precursor measurement by simple and rapid liquid chromatography-tandem mass spectrometry (Lc-Ms/Ms) method: comparison with current routine immunoassays

Fanelli Flaminia , Mezzullo Marco , Fazzini Alessia , Baccini Margherita , Gambineri Alessandra , Vicennati Valentina , Pelusi Carla , Pasquali Renato , Pagotto Uberto

Though LC-MS/MS is replacing immunoassays for major glucocorticoids and androgens routine assessment, the measurement of other sex steroids and precursors is still challenging and available only in experienced research laboratories. Our aim was to develop a simple-prep and rapid 2D-LC-MS/MS method for the simultaneous measurement of estrone (E1), estradiol (E2), dihydrotestosterone (DHT) and 17OHpregnenolone.Serum volumes of 300ul were spiked with intern...

ea0022p694 | Obesity | ECE2010

Reference interval values of four plasmatic endocannabinoids measured by isotopic dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)

Fanelli Flaminia , Cuomo Gaia , Di Lallo Valentina Diana , Belluomo Ilaria , Baccini Margherita , Gambineri Alessandra , Vicennati Valentina , Pasquali Renato , Pagotto Uberto

The endocannabinoid system is involved in modulating many metabolic and behavioural processes. Due to the instable nature of endocannabinoids in biological fluids and to the poor sensitivity of traditional analytical technologies, little is known about physiological circulating levels of these lipid mediators. We developed and validated an ID-LC-MS/MS method for the simultaneous measurement of four endocannabinoids: anandamide (AEA), 2-arachidonoylglycerol (2-AG), palmitoyl-et...

ea0022p740 | Steroid metabolism & action | ECE2010

Simultaneous steroid measurement by isotopic dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS): comparison with routine analysis methods and reference intervals in normal subjects

Fanelli Flaminia , Belluomo Ilaria , Di Lallo Valentina Diana , Cuomo Gaia , Baccini Margherita , Vicennati Valentina , Gambineri Alessandra , Pasquali Renato , Pagotto Uberto

The development of a reliable and rapid method to simultaneously measure serum steroids represents a compelling challenge for modern endocrinology. Immunoassays are very sensitive methods, being widely employed in automated routine laboratories, lacking, however, in specificity due to cross-reactivity and matrix interference. Gas chromatography–mass spectrometry (GC-MS) constitutes the reference method for quantitative analysis of steroids, but time-consuming sample pre-t...

ea0041ep784 | Obesity | ECE2016

The circulating fingerprint revealed by targeted metabolomic as biomarker of metabolic impairment in female overweight/obesity

Mezzullo Marco , Fanelli Flaminia , Fazzini Alessia , Baccini Margherita , Casadio Elena , Ibarra-Gasparini Daniela , Mazza Roberta , Vicennati Valentina , Fontanesi Luca , Pasquali Renato , Pagotto Uberto

The comprehension of the network of metabolic processes may help the understanding of the molecular pathways driving obesity and related complications. However this is a hard task due to the large number of actors and their complex interplay. We aimed at exploring by targeted metabolomic the circulating metabolite profile in lean (NW, n=42; BMI: 18.5–24.9 kg/m2) and age-matched overweight/obese (OB, n=37, BMI≥25.0 kg/m2), drug...