Searchable abstracts of presentations at key conferences in endocrinology

ea0014p222 | (1) | ECE2007

Human adipose tissue derived DPP-IV regulates lipolysis through NPY in cultured abdominal subcutaneous adipocytes

Kos Katarina , Baker Adam , Harte Alison , McTernan Philip , O’Hare Paul , Kumar Sudhesh

We have previously shown that the orexigenic hormone NPY is secreted by human adipocytes. The orexigenic hormone NPY(1–36) is truncated by the dipeptidyl-inhibitor IV (DPP-IV) to NPY(3–36) as consequence its affinity changes from receptor Y1 to Y4 and 5. The aim was to investigate whether DPP-IV is expressed in adipose tissue (AT) where it could modulate adipose tissue growth through modulation of NPY activity. This is relevant in light of DPP-IV inhibitors utilised ...

ea0015oc18 | Tumours, diabetes, bone | SFEBES2008

Human omental adipose tissue as a key site for the inflammatory 15-lipoxygenase pathway: Implications for metabolic disease

Hill Margaret J , McTernan Philip G , da Silva Nancy F , McGee Kirsty C , Kusminski Christine M , Baker Adam R , Kumar Sudhesh , Harte Alison L

Materials and methods: Paired AbSc and Om AT were collected from patients undergoing elective liposuction surgery (Age; 45±1.72 years; lean BMI; 22.91±0.56 kg/m2; obese BMI; 33.51±1.04 kg/m2; n=23) for gene expression analysis by microarray and real time polymerase chain reaction (PCR). Fasted blood was taken to measure metabolic parameters.Results: Microarray data showed that Om AT had signi...

ea0015oc21 | Tumours, diabetes, bone | SFEBES2008

Delineating the mechanisms of Visfatin regulation in human AT and the implications of TZD treatment in Type 2 Diabetes

McGee Kirsty , Harte Alison , da Silva Nancy , Creely Steven , Baker Adam , Kusminski Christine , Khanolkar Manish , Evans Marc , Chittari Madhu , Patel Vinod , Boardman Shirine , Kumar Sudhesh , McTernan Philip

Objective: The adipocytokine visfatin, expressed in abdominal adipose tissue (AT) is thought to mimic insulin activity. However, whilst central adiposity is closely related to insulin resistance (IR) and T2DM, visfatins’ role in the development of these conditions remains unclear.Method: We investigated circulating visfatin levels in non-diabetic (ND) and diabetic (T2DM) subjects and in T2DM patients pre- and post- rosiglitazone (RSG) treatment. We ...

ea0013p186 | Diabetes, metabolism and cardiovascular | SFEBES2007

Mitochondrial genes related to energy homoeostasis are dysregulated in obesity and type 2 diabetes in human adipose tissue

McGee Kirsty C , Hardo Faddy J , Baker Adam R , Tripathi Gyanendra , Creely Steven J , da Silva Nancy F , Kumar Sudhesh , Clapham John C , McTernan Philip G

Mitochondrial dysfunction has been implicated in the pathogenesis of obesity and Type 2 Diabetes Mellitus (T2DM). Recent research has investigated the role of mitochondria in skeletal muscle and suggesting mitochondrial dysregulation may be linked to T2DM. To date few studies have focused on the role of human adipose tissue (AT) in energy homoeostasis. Therefore we investigated a number of mitochondrial genes including PPARγ co-activator1 (PGC1α, mitochondrial biogen...

ea0013oc36 | British Thyroid Association Award | SFEBES2007

Comparsion of the NFκB and JNK mediated inflammatory mechanisms for production of adipocytokines in paired human epicardial and gluteo-femoral adipose tissue from CABG patients

Baker Adam R , Harte Alison L , da Silva Nancy F , Hill Margaret J , Ranasinghe Aaron , Khunti Kamlesh , Davies Melanie J , Pagano Dominico , Bonser Robert , Kumar Sudhesh , McTernan Philip G

Adipose tissue (AT) distribution impacts directly on type 2 diabetes and cardiovascular disease risk, however few studies have investigated the site-specific nature of human epicardial AT compared with gluteo-femoral (thigh) fat. Our recent studies have implicated epicardial AT as an important source of pro-inflammatory cytokines which may impact on myocardial function. Therefore the aim of this study was, using matched paired epicardial AT and gluteo-femoral (thigh) AT from p...

ea0013p183 | Diabetes, metabolism and cardiovascular | SFEBES2007

Depot-specific activity of NFκB and JNK in human abdominal subcutaneous and omental adipose tissue

da Silva Nancy F , Fowler Anne E , Harte Alison L , Baker Adam R , Kusminski Christine M , Creely Steven J , Starcynski Jane , O’Hare Joseph P , Kumar Sudhesh , McTernan Philip G

Central obesity is strongly associated with sub-clinical inflammation, insulin resistance (IR) and type 2 diabetes mellitus (T2DM), but the intracellular mechanisms involved in the pathogenesis of central obesity remain unclear. Recent studies have implicated NFκB and c-jun N terminal kinase (JNK) as central molecules linking insulin action and inflammation in mice. As such we examined (1) the inflammatory intracellular signaling pathways involving NFκB, JNK as a pot...