Searchable abstracts of presentations at key conferences in endocrinology

ea0065p229 | Metabolism and Obesity | SFEBES2019

Steroid deconjugation by helix pomatia – can we overcome snail speed?

Shaheen Fozia , Gilligan Lorna , Berner Camila , Callejas Jose Luis , Shackleton Cedric , Arlt Wiebke , Taylor Angela

Gas chromatography–mass spectrometry (GC–MS) has been used for urinary steroid analysis for over 50 years. The process of hydrolysis to release steroids from their conjugates is routinely performed using commercially available snail Helix-Pomatia sulfatase (HP). However, as this is a naturally occurring enzyme its production process results in batch variation and so each batch requires experimental validation to adjust for the difference in efficiency of the enzymati...