Searchable abstracts of presentations at key conferences in endocrinology

ea0016p658 | Signal transduction | ECE2008

G protein coupling and adenylyl cyclase inhibition are mediated by the DRY motif in the second intracellular domain of the SST5 receptor

Peverelli Erika , Lania Andrea , Mantovani Giovanna , Bondioni Sara , Beck-Peccoz Paolo , Spada Anna

Somatostatin exerts inhibitory effects on hormone secretion and cell proliferation by interacting with five different receptors (SST1–SST5) linked to effector systems via G proteins. The receptor structural domains mediating these effects have not been identified. Since SS is the hypothalamic peptide that physiologically inhibits GH secretion, aim of this study was to investigate the molecular determinants mediating the interaction of the human SST5 with G proteins and su...

ea0016p588 | Paediatric endocrinology | ECE2008

Epigenetic defects at GNAS DMRs in PHP-Ia patients lacking coding GNAS mutations

Bondioni Sara , Mantovani Giovanna , Labarile Pamela , de Sanctis Luisa , Peverelli Erika , Lania Andrea , Beck-Peccoz Paolo , Spada Anna

Pseudohypoparathyroidism (PHP) is a disorder characterized by hypocalcemia and hyperphosphatemia due to end-organ resistance to the action of PTH. The two main subtypes of PHP, PHP type Ia and Ib are caused by heterozygous loss-of-function mutations in GNAS exons 1–13, which encode Gsα, and by methylation defects in the imprinted GNAS cluster, respectively. Individuals affected with PHP-Ia typically show clinical abnormalities referred to as Albright hereditary osteo...

ea0014oc9.5 | Signal transduction | ECE2007

The third intracellular loop of human SST5 is crucial for receptor internalization after SS28 stimulation

Peverelli Erika , Mantovani Giovanna , Lania Andrea G , Calebiro Davide , Doni Andrea1 , Bondioni Sara , Beck-Peccoz Paolo , Spada Anna

Somatostatin (SS) is a widely distributed polypeptide that exerts inhibitory effects on hormone secretion and cell proliferation by interacting with five different receptors (SST1-SST5), that display important differences in tissue distribution, coupling to second messengers, affinity for SS and intracellular trafficking. SS analogues currently used in the treatment of acromegaly inhibit hormone secretion and cell proliferation by binding to SST2 and 5. Beta-arrestins have bee...

ea0014p200 | (1) | ECE2007

Expression of PKA regulatory subunits inversely correlates with BMI and insulin resistance parameters in human adipocytes from lean and obese subjects

Bondioni Sara , Mantovani Giovanna , Alberti Luisella , Corbetta Sabrina , Gilardini Luisa , Invitti Cecilia , Zappa Marco Antonio , Ferrero Stefano , Peverelli Erika , Lania Andrea G , Beck-Peccoz Paolo , Spada Anna

In human adipocytes the cAMP-dependent pathway mediates signals originating from the activation of beta-adrenergic receptors, thus playing a key role in the regulation of important metabolic processes such as lipolysis and thermogenesis. CyclicAMP effects are mainly mediated by cAMP-dependent protein kinase (PKA), a tetrameric enzyme composed by two catalytic subunits associated with two regulatory (R) subunits. There are four different R subunit genes and proteins (R1A, R1B, ...