Searchable abstracts of presentations at key conferences in endocrinology

ea0065p166 | Metabolism and Obesity | SFEBES2019

Obesity-induced changes in hepatocyte and skeletal myocyte expression of mRNAs encoding islet GPCR peptide ligands

Atanes Patricio , Smith Lorna , Bowe James , Persaud Shanta

Introduction: Insulin-sensitive tissues such as liver and skeletal muscle modify their gene expression under conditions of obesity-induced insulin resistance, and some of these gene products may be released to maintain glucose homeostasis. This study aimed to identify mRNAs encoding liver and skeletal muscle peptides that have the potential to regulate β-cell function by binding to islet GPCRs, and to quantify changes in expression of these liver and muscle mRNAs in obese...

ea0065p200 | Metabolism and Obesity | SFEBES2019

A role for kisspeptin in long-term islet function in females

Smith Lorna , Hill Thomas , Simpson Sian , Jones Peter , Bowe James

Although characterised predominantly for its role in the brain, the neuropeptide kisspeptin has previously been shown to potentiate glucose-stimulated insulin through its receptor, GPR54; expressed abundantly in β-cells. We have previously reported a physiological role for kisspeptin signalling in the islet adaptation to pregnancy, using a β-cell specific GPR54 knockout mouse line (βGPR54ko). The aim of the present study was to examine the effects of impaired &#...

ea0065p346 | Reproductive Endocrinology and Biology | SFEBES2019

Islet adaptations to pregnancy: a role for Urocortin 2

Simpson Sian , Smith Lorna , Jones Peter , Bowe James

Previous studies have shown beneficial effects of the corticotropin releasing hormone (CRH) family, including the urocortins (UCN1, UCN2 and UCN3), on pancreatic islets and subsequent glucose homeostasis. However, the physiological relevance of this interaction is not currently understood. CRH and urocortins are also expressed by placenta, so this study investigated whether signalling through CRH receptor 1 or 2 (CRHR1/CRHR2) plays a role in the islet adaptation to pregnancy. ...

ea0077lb44 | Late Breaking | SFEBES2021

Depression and islet function during pregnancy: Generation of a depressive phenotype using UCMS

Smith Lorna , Fernandes Cathy , Simpson Sian , Liu Bo , Jones Peter , Bowe James

Gestational diabetes (GDM) occurs when beta-cell insulin secretory capacity is insufficient to meet the increased demands required to maintain normoglycemia during pregnancy. Considerable clinical evidence supports a link between depression and GDM, although underlying mechanisms are unclear. We used the unpredictable chronic mild stress (UCMS) rodent model of depression to examine the metabolic effects of depression in pregnant mice. C57BL/6J females were divided at 4-weeks-o...

ea0086p203 | Metabolism, Obesity and Diabetes | SFEBES2022

Monitoring obesity-induced hyperglycaemia and insulin sensitivity in obese murine pregnancy

Morris Jessica , Kennard Matilda , Farrel-Dillon Keith , Taylor Paul , Bowe James , Gnudi Luigi , Nandi Manasi , Chapple Sarah

During pregnancy progressive maternal insulin resistance occurs which is normal, ‘sparing’ glucose that can be transported across the placenta to be used by the fetus. In gestational diabetes mellitus (GDM) maternal glucose dysregulation is exacerbated, characterised by hyperglycaemia and associated with long-term adverse outcomes in mother and child, such as increased risk of diabetes and/or cardiovascular disease in later-life. Continuous glucose monitoring (CGM) i...

ea0065p181 | Metabolism and Obesity | SFEBES2019

The progesterone metabolite epiallopregnanolone sulphate induces glucose-stimulated insulin secretion from human and mouse islets and is reduced in gestational diabetes mellitus

Fan Hei Man , Bellafante Elena , Mitchell Alice , Ovadia Caroline , Martineau Marcus , Jones Peter , McCance David , Marschall Hanns-Ulrich , Hunt Kate , Bewick Gavin , Bowe James , Williamson Catherine

Serum concentrations of progesterone sulphates are raised in intrahepatic cholestasis of pregnancy (ICP), the commonest pregnancy-specific liver disease. Women with ICP have increased rates of gestational diabetes mellitus (GDM). We hypothesised that raised progesterone sulphates may modulate glucose homeostasis. Progesterone sulphates were assayed in serum samples from participants of the hyperglycaemia and adverse pregnancy outcomes (HAPO) study (n=79–94), and ...