Searchable abstracts of presentations at key conferences in endocrinology

ea0020p553 | Neuroendocrinology, Pituitary and Behaviour | ECE2009

Ghrelin as new provocative test for the diagnosis of GH deficiency in adults

Gasco Valentina , Rovere Silvia , Beccuti Guglielmo , Broglio Fabio , Aimaretti Gianluca , Grottoli Silvia , Casanueva Felipe , Ghigo Ezio

ITT is the test of reference for the diagnosis of adult GH deficiency (GHD), but it is recognized that also GHRH in combination with arginine (ARG) or GH secretagogues (GHS) are equally reliable tests. It has also been suggested that testing with GHS would represent a potent stimulus exploring also the integrity of hypothalamic pathways controlling somatotropic function. We therefore aimed to clarify the diagnostic reliability of testing with ghrelin, the natural GHS. We studi...

ea0014p575 | (1) | ECE2007

The GH releasing activity of ghrelin is insensitive to the negative growth hormone (GH) autofeedback in humans

Riganti Fabrizio , Prodam Flavia , Gramaglia Elena , Benso Andrea , Lucatello Barbara , Ghigo Ezio , Broglio Fabio

Growth hormone (GH) secretion is regulated by a complex interplay between GH-releasing hormone (GHRH), somatostatin and several other central and peripheral modulatory signals. Ghrelin has been hypothesized as physiological amplifier of GH pulsatility and acts via mechanisms, at least partially, independent of GHRH and somatostatin. The GH response to GHRH is strongly inhibited by previous administration of recombinant human GH (rhGH), likely as a consequence of a somatostatin...

ea0026p710 | Diabetes (epidemiology, pathophysiology) | ECE2011

Continuous glucose monitoring enhances the diagnostic value of mixed meal test in reactive hypoglycemia

Olivetti Ilaria , Grassi Giorgio , Lambertenghi Veronica , Tomelini Michela , Gigliardi Valentina Ramella , Gramaglia Elena , Ghigo Ezio , Broglio Fabio

Background: Clinical reactive hypoglycemic events are defined as the coexistence of plasma glucose <60 mg/dl and autonomic and/or neuroglycopenic symptoms occurring in fed conditions. The diagnosis is based on tests aiming to simulate the causative triggers (i.e. mixed meal test). In clinical practice, however, a high percentage of symptomatic patients results negative to such diagnostic approach, leading to the impression of an inadequate sensitivity of this diagnostic to...

ea0022p601 | Neuroendocrinology and Pituitary (<emphasis role="italic">Generously supported by Novartis</emphasis>) | ECE2010

Basal and stimulated GH secretion in Cushing's syndrome: effects of ghrelin and GHRH+arginine

Picu Andreea , Marinazzo Elisa , Prodam Flavia , Broglio Fabio , Belcastro Sara , Aimaretti Gianluca , Giordano Roberta , Ghigo Ezio , Arvat Emanuela

GH secretion is usually impaired in active Cushing’s syndrome (CS), due to concomitant mechanisms, including diminished GHRH and/or increased somatostatin release and impaired pituitary somatotrope responsiveness. Differently, IGF1 levels not parallel GH insufficiency in CS, being reported reduced, normal or increased. Both ghrelin and GHRH+arginine (ARG) are powerful GH secretagogues, influenced by age and/or BMI. In 27 CS (42.7±2.9 years, 28.8±0.9 kg/m2</...

ea0020oc4.5 | Acromegaly/IGF1/Type 2 Diabetes | ECE2009

Regulation of obestatin levels during unacylated ghrelin infusions in normal and type 2 diabetic patients

St-Pierre David H , Settanni Fabio , Olivetti Ilaria , Gramaglia Elena , Benso Andrea , Riganti Fabrizio , Ghigo Ezio , Broglio Fabio

Background: Obestatin, a ghrelin gene product was recently isolated but important questions remain regarding its regulation and its physiological effects. The aim of the present study was to evaluate the effect of unacylated ghrelin (UAG) on obestatin levels in normal subjects and patients with type 2 diabetes (T2D).Methods: Five normal subjects and 5 T2D patients were included in this study. Unacylated ghrelin (3 μg/kg per hour) or saline were cont...

ea0020p117 | Thyroid | ECE2009

Subjective and objective sleep evaluation in patients with hypo- and hyper-thyroidism

Riganti Fabrizio , Noi Fabiana Di , Seardo Maria Angela , Gramaglia Elena , Bonelli Nadia , Rossetto Ruth , Ghigo Ezio , Broglio Fabio

Hyper- and hypo-thyroidism are considered as clinical conditions of sleep alterations. At present, however, these clinical reports have never been confirmed by studies providing a structured description of subjective and objective sleep quantity and quality. To this aim, we enrolled 15 patients with naïve overt hyperthyroidism (HYPER), 9 with naïve overt primary hypothyroidism (HYPO) and 15 healthy age-, sex- and BMI-matched control subjects (CS). Clinical conditions...

ea0020p439 | Obesity and Metabolism | ECE2009

The evaluation of metabolic effects following ghrelin and salbutamol administration

Benso Andrea , St-Pierre David H , Gramaglia Elena , Riganti Fabrizio , Lucatello Barbara , Olivetti Ilaria , Ghigo Ezio , Broglio Fabio

Background: The activation of the GHS-R1a receptor by acylated ghrelin (AG) or by synthetic GH secretagogues (GHS), potently stimulates GH release and mediates other neuroendocrine/metabolic effects. Beta-adrenergic receptor agonists negatively influence GH secretion and other metabolic functions. The GH response to AG and GHS is refractory to the inhibitory effect of β2-adrenoceptors activation but no other report has evaluated the interactions between AG and salbutamol ...

ea0016p512 | Obesity | ECE2008

No feedback inhibition of obestatin during acylated ghrelin infusion in humans

Lucatello Barbara , St-Pierre David H. , Settanni Fabio , Olivetti Ilaria , Benso Andrea , Granata Riccarda , Ghigo Ezio , Broglio Fabio

Background: Obestatin is a 27 aa peptide derived from the ghrelin gene. Obestatin was first described to exert anorexigenic effects by decreasing gastric motility in rodent models, however these results have been debated and there is presently only small information regarding its activity and its regulation in humans. Furthermore, its interactions with acylated ghrelin (AG), derived from the same gene, have not been evaluated. Therefore, in these preliminary data, we sought to...

ea0016p532 | Obesity | ECE2008

Diabetogenic effects of a continuous infusion of acylated ghrelin in normal and type 2 diabetic patients

St-Pierre David H , Olivetti Ilaria , Pomero Elisa , Gramaglia Elena , Riganti Fabrizio , Benso Andrea , Ghigo Ezio , Broglio Fabio

Background: Previous studies have shown that acylated ghrelin (AG) modulates insulin secretion and glucose disposal in vitro and after acute administration in humans. The aim of the present study was to evaluate postprandial glucose and insulin response following AG infusion in normal subjects and patients with type 2 diabetes.Methods: Six normal subjects and 3 patients with type 2 diabetes were included in this study. AG (1 μg/kg per h) or s...

ea0014p622 | (1) | ECE2007

Ghrelin effects on spontaneous and stimulated LH secretion in human males

Bonelli Lorenza , Baldi Matteo , Broglio Fabio , Bertagna Angela , Di Bisceglie Cataldo , Tagliabue Milena , Manieri Chiara , Ghigo Ezio , Lanfranco Fabio

Acylated ghrelin (AG) participates in the modulation of the hypothalamic-pituitary-gonadal axis functions, with a predominantly inhibitory effect upon the reproductive system in animals. Animal studies have shown that ghrelin suppresses LH secretion in vivo, decreases LH responsiveness to GnRH in vitro and partially delays the timing of puberty in males. Aim of this study was to evaluate the effects of AG infusion on spontaneous and stimulated gonadotropin secret...