Searchable abstracts of presentations at key conferences in endocrinology

ea0086op4.2 | Metabolism, Obesity and Diabetes | SFEBES2022

Differential effects of L- and D-lactate on HCAR1 signalling

Milner Annabelle , Brown Alastair , Frost Gary , Hanyaloglu Aylin

Lactate is a metabolite that activates the G-protein coupled receptor, Hydroxycarboxylic acid Receptor 1 (HCAR1) to regulate physiological processes such as lipolysis, cancer cell survival, and neuroprotection. Lactate exists in two forms, L+ and D-, with the L isoform predominant in the human body. Interestingly, both isoforms are only found together in the gastrointestinal tract. L-lactate is synthesised as a by-product of anaerobic respiration, whereas D-lactate, is a produ...

ea0094p62 | Metabolism, Obesity and Diabetes | SFEBES2023

Metabolite-dependent regulation of HCAR1 at a spatial level and its potential to influence local gut barrier integrity

Milner Annabelle , Brown Alastair , Frost Gary , Hanyaloglu Aylin

The Hydroxycarboxylic Acid Receptor 1 (HCAR1) is a Gαi/o-coupled GPCR known to be activated by both L- and D-lactate; metabolites produced by gut microbiota and anaerobic metabolism. Elevated D-lactate levels are indicative of dysbiosis and inflammatory bowel disease. As lactate can be transported into cells, it has the potential to activate HCAR1 at the plasma membrane and within intracellular locations resulting in diversified responses. Whether HCAR1 signalling is spat...

ea0077lb40 | Late Breaking | SFEBES2021

Novel ultrasound approaches permit the visualisation of the microvascular effects of glucagon-like peptide-2 in the gut with unprecedented resolution

Broughton-Venner Jacob , Roberts Anna , Norton Mariana , Phuah Phyllis , Brown Alastair , Tang Mengxing , Murphy Kevin

The screening and monitoring of intestinal diseases still provides challenges within in vivo and clinical research. For example, patients with Crohn’s disease will typically have a biannual endoscopic examination – this invasive procedure is distressing for patients and hence its frequency is limited at the expense of sufficient monitoring of pathological progression. Modalities such as CT and MRI can image the GI tract, however, they are ionising, which nor...

ea0086p64 | Metabolism, Obesity and Diabetes | SFEBES2022

Novel contrast enhanced ultrasound imaging approaches to understand and treat gastrointestinal disease

Dunsterville Cecilia , Vie Clotilde , Broughton-Venner Jacob , Tang Meng-Xing , Murphy Kevin , Brown Alastair

About one in six hospital admissions are for a primary diagnosis of gastrointestinal (GI) disease in the UK, according to the British Society of Gastroenterology, and there is a similar incidence of disease in other developed countries. Inflammatory disease of the gut, in particular, alters the release of cytokines and gut hormones. Medication is used to manage GI diseases and endoscopies help visually monitor its progression. However, this procedure is invasive and causes dis...

ea0094p213 | Metabolism, Obesity and Diabetes | SFEBES2023

Super-resolution ultrasound imaging approaches to visualise changes in gut structure

Dunsterville Cecilia , Vie Clotilde , Broughton Venner Jacob , Yan Jipeng , JH Brown Alastair , Tang Meng-Xing , G Murphy Kevin

The gut plays an important role in energy homeostasis, and gut function is under intensive neuroendocrine control. The non-invasive imaging techniques contrast enhanced ultrasound (CEUS) imaging and super resolution ultrasound (SRUS) have the potential visualise the gut and provide novel insight into its structure and function. We aimed to demonstrate that changes in duodenal villi structure can be monitored using CEUS and SRUS, providing an insight into the structural of the ...