Searchable abstracts of presentations at key conferences in endocrinology

ea0037ep1121 | Endocrine tumours | ECE2015

Identification of human SST2 somatostatin receptor domains involved in receptor internalization and signaling in pancreatic neuroendocrine tumors

Cambiaghi Valeria , Vitali Eleonora , Mantovani Giovanna , Spada Anna , Peverelli Erika , Lania Andrea

Somatostatin exerts its inhibitory effects on hormone secretion and cell proliferation via five receptors subtypes (SST1-SST5). After agonist binding, receptor residues mainly located in the carboxyl terminal (CT) and in the third intracellular loop (IC3) are phosphorylated and β-arrestins are recruited to drive SSTRs internalization.Aim of the study is to characterize the intracellular mechanisms responsible for SST2 internalization and identify th...

ea0037gp.28.05 | Endocrine tumours and neoplasia – NETS | ECE2015

Filamin-A is involved in stabilisation, signal transduction, and angiogenesis regulation mediated by somatostatin receptor 2 in pancreatic neuroendocrine tumors

Vitali Eleonora , Cambiaghi Valeria , Loiarro Federica , Zerbi Alessandro , Colombo Piergiuseppe , Spada Anna , Mantovani Giovanna , Peverelli Erika , Carnaghi Carlo , Lania Andrea

Somatostatin receptor type 2 (SST2) is the main pharmacological target of long-acting somatostatin analogues (SSA) widely used in patients with pancreatic neuroendocrine tumours (P-NETs). A subset of patients is resistant to SSA, although the molecular mechanisms responsible for resistance are poorly understood. Several studies identified cytoskeleton protein interactions as determinant in receptor anchoring, expression and signalling. Since SST2 was recently demonstrated to a...

ea0035p517 | Endocrine tumours and neoplasia | ECE2014

The effects of cAMP in different neuroendocrine tumorous cells: the role of Epac and PKA in cell proliferation and cell adhesion

Vitali Eleonora , Cambiaghi Valeria , Zerbi Alessandro , Peverelli Erika , Mantovani Giovanna , Loiarro Federica , Carnaghi Carlo , Spada Anna , Lania Andrea

cAMP is an ubiquitous second messenger that is implicated in the regulation of a wide variety of cell functions, including cell proliferation that is differently affected depending on the cell type. Although the effects exerted by cAMP were initially attributed to PKA activation, two exchange proteins directly activated by cAMP (Epac1/2) have been identified as cAMP targets able to mediate several cAMP effects. Aim of this study was to investigate the effect of cAMP on neuroen...

ea0032p824 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

Role of filamin-A in the regulation of SST2 receptor localization and signalling in tumoral GH-secreting cells

Giardino Elena , Peverelli Erika , Vitali Eleonora , Cambiaghi Valeria , Lania Andrea G , Peccoz Paolo Beck , Mantovani Giovanna , Spada Anna

Somatostatin (SS) binds to different SS receptors (SSTR1–5) and SS analogues are the first choice medical treatment of GH-secreting pituitary adenomas (GH-omas). A subset of patients is resistant to SS, although the mechanisms involved in SS resistance are not fully understood. Recent studies identified specific protein–protein interactions as determinant in the regulation of receptor anchoring and signalling. Filamin A (FLNA) is a widely expressed cytoskeleton prote...